ASSESSMENT OF THE SENSITIVITY OF REAL-TIME PCR FOR DIAGNOSIS OF NEWCASTLE DISEASE VIRUS

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SATTARI S.; BANABAZI M.H.; VAKOOHI SH.; TABATABAEI M.

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Paper Information

Journal: Veterinary Research & Biological Products Year:2016 | Volume:29 | Issue:1 (110) Page(s): 66-71

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Title

ASSESSMENT OF THE SENSITIVITY OF REAL-TIME PCR FOR DIAGNOSIS OF NEWCASTLE DISEASE VIRUS

Author(s)

SATTARI S. | BANABAZI M.H. | VAKOOHI SH. | TABATABAEI M. | Issue Writer Certificate

Keywords

NEWCASTLE DISEASE VIRUS (NDV)Q1

DIAGNOSISQ2

REAL-TIME PCRQ1

SENSITIVITYQ1

Abstract

Newcastle disease is one of the most serious viral diseases in the poultry worldwide. Since the traditional strategies have been not control it well, the aim of this study was to introduce new methods for early and rapid DIAGNOSIS of Newcastle.In this study, to detect the virus, a REAL-TIME PCR method was optimized, Viral RNA was extracted from strain B1 using the kit RNease mini (Qiagen, USA), according to the manufacturer's instructions. This sample had 68.23×109 copy numbers of viral RNA per each microliter. Then, a serial dilution as 100-fold was prepare from the initial sample. Then, these dilutions were simultaneously applied in reverse transcription and Realtime PCR using commercial kits (Genekam Biotechnology, Germany) according to the manufacture’s instruction. The SENSITIVITY of REAL-TIME PCR reaction was determined based on serial dilutions of 1×10-34 copy number per micro liter. Since, speed and accuracy in DIAGNOSIS of contagious of Newcastle disease virus plays an important role to control the disease, so adopting this method is recommended as a diagnostic test with high SENSITIVITY.

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APA: Copy

SATTARI, S., BANABAZI, M.H., VAKOOHI, SH., & TABATABAEI, M.. (2016). ASSESSMENT OF THE SENSITIVITY OF REAL-TIME PCR FOR DIAGNOSIS OF NEWCASTLE DISEASE VIRUS. VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI), 29(1 (110)), 66-71. SID. https://sid.ir/paper/201061/en

Vancouver: Copy

SATTARI S., BANABAZI M.H., VAKOOHI SH., TABATABAEI M.. ASSESSMENT OF THE SENSITIVITY OF REAL-TIME PCR FOR DIAGNOSIS OF NEWCASTLE DISEASE VIRUS. VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI)[Internet]. 2016;29(1 (110)):66-71. Available from: https://sid.ir/paper/201061/en

IEEE: Copy

S. SATTARI, M.H. BANABAZI, SH. VAKOOHI, and M. TABATABAEI, “ASSESSMENT OF THE SENSITIVITY OF REAL-TIME PCR FOR DIAGNOSIS OF NEWCASTLE DISEASE VIRUS,” VETERINARY RESEARCHES BIOLOGICAL PRODUCTS (PAJOUHESH-VA-SAZANDEGI), vol. 29, no. 1 (110), pp. 66–71, 2016, [Online]. Available: https://sid.ir/paper/201061/en

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