Nano particles-based catalysts are additives that can be used in green fuels to improve the characteristics of an engine. In the present study, non-edible Aegle marmelos (AM) de-oiled seed cake biomass is used for the production of pyrolysis oil. The copper oxide (CuO) nanoparticles are prepared via the Sol-Gel process and categorized by Scanning Electron Microscopy (SEM), Energy-Dispersive X-Ray Analysis (EDAX), and Fourier Transform InfraRed (FT-IR) Spectroscopy. The CuO nano particles are mixed with pyrolysis oil-diesel opus at a fraction of 30 and 50 ppm. The Technique for Order Preference by Similarity to the Ideal Solution (TOPSIS) tool is employed to attain the optimum engine process parameters. The correlation between cylinder head temperature and exhaust emission was concluded by using an innovative approach to thermography image processing. The outcomes of experimental, TOPSIS, and image processing revealed that modified fuel opus emitted a lesser amount of carbon monoxide, oxides of nitrogen, and unburned hydrocarbon compared with diesel but augmented CO2 (Carbon dioxide) emission. It is found that higher CO2 emission is apparent for the dynamic combustion process. This study confirms that AM bio-oil opus is an affordable alternate fuel for diesel engines for a clean and green environment.