The effect of concentrations of egg yolk (2.5, 5, 7.5, 10, 15 and 20%) in Tris diluent on viability, motility and progressive motility of frozen-thawed Markhoz goat in breeding season was studied. The semen was collected from 5 Markhoz bucks twice a week during 7 weeks. Every time after collecting Fresh semen evaluated for motility and sperm concentration. Semen diluted with Tris-Citric acid- Fructose (TCF) extender containing different concentration egg yolk was cooled to 5oc and then frozen at-80oc in nitrogen vapor and stored it in liquid nitrogen. Thawing was performed by placing the straws in a water bath at 37oC for 30s. The post-thaw viability was 57.48, 60.17, 55.21, 54.91, 53.74 and 49.53% for 2.5, 5, 7.5, 10, 15 and 20% of egg yolk concentration, respectively. The effects of different concentrations of egg yolk on post-thaw motility were as 52.92, 55.71, 50.78, 49.85, 49.21 and 45.35%, respectively. The results for post-thaw progressive motility showed 39.21, 42.28, 37.85, 36, 37 and 32.42% in different concentrations of egg yolk, respectively. Maximum and minimum of viability, motility and progressive motility were obtained in 5 and 20% egg yolk concentration, respectively. The result show a significant decrease in sperm viability and motility by increasing the egg yolk in Tris buffer (P<0.05). Therefore, using 5% of egg yolk concentration in Tris diluent can be suitable for cryopreservation sperm Markhoz goat during breeding season.