Characterization studies and determination of liberation degree are first stages of mineral processing. These studies are very important for determination of optimum comminution conditions, selection of perspiration process and determination of suitable processing methods. Khoshoomi mine with the average uranium grade of 1540 ppm, is located at 150km northeast of Yazd. The characterization studies of this ore are made by XRD method and microscopic study, also degree of liberation studies are made by microscopic section. These studies are done by three powder samples, twelve thin sections and twelve polished sections. The major minerals such as Feldspar (Albite), Plagioclase (Orthoclase), Biotite, Chlorite, Carbonate and minor minerals such as Sphen, Zrconium, Actinolite, Tremolite, Pitchblende and Betafite were identified based on the results of these studies. Accordingly, by microscopic studies and heavy media method, the liberation degree of Orthoclase, Albite, Biotite and Uranium minerals were determined as 320, 290, 190 and 110 micron, respectively.