Background and Aims: swimming pool is one of the most attractive and popular sports centers for people. Pools hygiene to protect the swimmers’ health has a special importance whereas neglecting the health rules and standards can lead to various diseases. This study aimed to investigate the microbial contamination and disinfection methods in the Sabzevar- Jovein swimming pools.Materials and Method: This is a descriptive cross-sectional study to assess the microbial situation and disinfection methodes in Sabzevar and Jovin pools 127 samples out of 7 pools were collected (6 pools in Sabzevarand1pool in Jovein) over a12 months period. Studied parameters included bacteria E. Coli, total coli form heterotrophic plate counted (HPC) as well as residual chlorine, pH, and temperature. The results were compared with the standards implying the spss software and the data analysis were commited based on the Pearson and Spearman correlation coefficient.Results: According the results of this study the PH, chlorine residual, E. Coli, total coliform and heterotrophic bacteria, were respectively, 16.40, 12.55, 29.6, 90.18, 95.40% each of which exceeded the standards.Conclusion: In general, swimming pools in Sabzevar showed the average condition in terms of hygiene status while swimming pool in Jove in was in a suitable condition. Accordingly, the necessary measures for a proper sterilization should be considered in these places. Moreover, the users instruction, especially bathing before entering the water which plays an important role in preventing staphylococcal infection, should be emphasized.