Background and objectives: Drought stress is major constrain for crop production worldwide and is the common stress. Natural fertilizer and humic acide application causes consume less water, lower water evaporation from soil surface and water storage in soil. So, because of importance of portulaca for hailing of cardiovascular disease, cancer, asthma, Diabaties and infectious diseases an experiment conducted for evaluation of Humic acid on some of morphological and physiological charactristics of portulaca oleracea under water stress.Materials and Methods: In order to investigate the effects of Irrigation regims and Humic acid levels on morphophysiological traits on portulaca (Portulaca oleracea L.), as a medicinal plant a pot experiment was conducted as factorial based on completely randomized design with three replications at Gorgan University of Agricultural Sciences and Natural Resources in 2014. Treatments included irrigation regimes in four levels (100, 75, 50 and 25% FC) and humid acid in four levels (0, 200, 400 and 600 mg L-1). Studied traits included the water stress reduced shoot length, number of stem, root diameter, root length, stem wet and dry weight, leaf wet and dry weight, leaf area index, proline, leaf water ratio and a, b and total chlorophyll content. Chlorophyll and Proline were measured with Arnon and Bates method.Results: The results showed that by increasing drought stress, shoot length, number of stem, root length, stem wet and dry weight, leaf wet and dry weight, leaf area index were decreased. Also, with increasing drought stress, leaf water ratio and a, b and total chlorophyll content decreased and proline were increased in comparison with control. Choloropyll a, b and total decreased with irrigation regimes and at the lowest rate at 25% FC was 0.40, 0.25 and 0.66 mg g-1, respectively. Humic acid had significant effects on all of morphological and physiological characteristic except of stem wet and dry weight and the highest rate was observed at 200 mg L-1. Humic acide effect on proline and relative water content was significant but on cholorophyll a, b and total was not significant. Intraction effects of Irrigation regime and humic acid had significant effects on all of morphological and physiological characteristic except of stem wet and dry weight, leaf area index and b chlorophyll content. Proline increased compared to control (1.72%) in 600 mg L-1 humic acid. The highest value of a and total chlorophyll content was obtained in 100% FC and 200 mg L-1 humic acid. The most value of LWR was meatured in 100% FC and 400 mg L-1 humic acid.Conclusion: The results showed that by increasing drought stress, all of the measured traits were decreased, In spite of that, humic acid protected purslane against drought and decreased drought damages. The best treatment was 75% FC and 200 mg L-1 humic acid in terms of economic benefit, Because of consuming less water and humic acide produce more yield. In general, with supplemental experiment humic acid could be applied for reducing adverse effect of irrigation regime.