Stress environment of space flights including hyper gravity and microgravity effect cell functions and processes. We studied effect of hyper gravity and flight stress on function, death, growth and cellular damage of five cell lines including human vascular endothelial cells, rat bone marrow stromal cells, mouse embryonic fibroblast cells, PC12 cells and pancreas islands in Biological payload of 3th sounding rocket by NO, LDH, MTT and insulin assay kits. Our results indicated that effect of hyper gravity increase significantly cellular death and NO secretion but we found no changes in insulin secretion and pancreas function.