Objectives: Considering the importance of assessing aspects of wellbeing in individuals and its role in physical and psychological health, this study was carried out to examine the reliability and validity of Ryff’s Psychological Well-being Scales (RPWBS).Method: In a descriptive cross-sectional study 145 students of Islamic Azad University, Azadshahr Branch (96 women and 49 men) who were selected using random stratified method, completed RPWBS, Self-acceptance subscales, Oxford Happiness Scale, and Rosenberg’s Self-esteem Scale. Data were analyzed using descriptive statistic methods and correlation coefficient.Results: The test-retest reliability coefficient of RPWBS was 0.82. The subscales of Self-acceptance, Positive Relation with Others, Autonomy, Environmental Mastery, Purpose in Life, and Personal Growth were found to be 0.71, 0.77, 0.78, 0.77, 0.70, and 0.78 respectively, which were statistically significant (p<0.001). The correlation coefficient of RPWBS with Satisfaction with Life, Happiness, and Self-esteem were also found to be: 0.47, 0.58, and 0.46 respectively which were also significant (P<0.001).Conclusion: The RPWBS are valid and reliable and are suitable for use in assessing the psychological well-being of Iranian students.