Background: Cardiovascular disease (CVD) and inflammation areamongthe mostcommoncauses of mortality in dialysis patients. Factors such as chronic inflammation, dyslipidemia and elevated homocysteine levels may underlie the increased risk of CVD. Objectives: This study aimed to investigate levels of inflammatory markers, homocysteine, lipid profile, WBC, and BMI levels in hemodialysis patients compared with the control subjects in Isfahan. Methods: A total of 32 hemodialysis patients and 32 healthy subjects were selected randomly and their serum levels of IL-6, CRP, homocysteine, and lipid profiles were measured. Results: According to the results, most of the studied hemodialysis patients compared to the healthy subjects had non-normal amounts of IL-6, CRP, and homocysteine i. e., higher than healthy subjects (P < 0. 05). Cholesterol, HDL, and LDL were significantly lower than the healthy subjects (P < 0. 05), while triglycerides and VLDL levels showed no significant difference (P: 0. 38 > 0. 05). A significant difference of BMI was observed between the normal subjects and the patients; thus, this factor in patients was less than what was in the healthy group (P: 0. 001 < 0. 05). Conclusions: The results demonstrated that in most patients the levels of factors IL-6, CRP, and homocysteine were abnormal compared to the healthy group. In addition, the risk of developing cardiovascular and inflammatory disease as well as dyslipidemia in these patients is higher than normal.