This study was carried out at Research center of farahan university in order to compare tolerance chilling stress and grain yield in prechilling spring cultivars with winter safflower cultivars in Markazi province in 2006. A randomized complete block design with three replications was used with 10 prechilling spring cultivars and 18 winter safflower cultivars: Padide, S-6-46, LRV-51-13, LRV-51-20, 697, LRV-290, K.W.2, Yenice, LRV-51-11, K.C.72, K.A.72, IL-128, Marand local variety, L.S.P, IL-119, IL-111, Miandoab local, Gila, uc-1, Nebraska-10, Esfahan local variety, Arak-2811, FO2, Davari-2811, ES.68, IL-111(2), LSP(2), LRV-51-51. Each plot consisted of 2 rows, 5 m long with 50cm between rows and 5cm between plants on the rows. Some important traits such as plant height, umber of branches per plant, number of grain per head, 1000-seed weight and grain yield were recorded. The results indicated that cultivar 697with 2691 kg/ha cultivar, LRV-51-20 with 2062 kg/ha and cultivar and, K.C.72 with 2043 kg/ha grain yield were significantly superior to the other treatments. The purpose of use prechilling spring cultivars to define least on new cultivar for planting in autumn in climate Farahan region.