Mineral accumulation and salinity increase osmotic pressure of soil solution and decrease the free energy of water, while plants alleviate this phenomenon by osmotic adjustment. In this situation, plants try to adjust their osmotic potential by accumulating N- compounds such as proline and OH-compounds in cell cytoplasm A such, part of photosynthetic products and ion accumulation needed for osmotic adjustment is consumed, thereby decreasing ion concentration needed for growth and yield of plants. Nowadays, biological methods are used to enable plants against salinity condition. In order to evaluate the role of mycorrhizal symbiosis in this respect, a completely randomized experimental design was set up in greenhouse condition with three levels of salinity i.e.4, 8, 12 dS/m using NaCl and CaCl2 in equal molar ratio, 5 inoculation treatments of mycorrhizal fungi including Glomus mosseae, Glomus intraradices, Glomus etunicatum, and a mixture of the mentioned species and check plants without inoculation, and two wheat cultivars, namely, Chamran and the promising Line 9 (Bank"S"/vee" S") as salininty semi tolerant and tolerant cultivars, respectively. Results showed that by increasing the salinity level, proline accumulation increased in wheat flag leaves. In Chamran cultivar, this concentration was higher than that in Line 9. On the other hand, water use efficiency, K, N, P, and Ca uptake in line 9 were higher than in Chamran cultivar. The difference between inoculated and un-inoculated treatments in Chamran cultivar and line 9 were significant for all the measured indicators. Mixed fungal treatment was more effective than the other fungal treatments in decreasing proline accumulation as well as increasing water use efficiency and mineral uptake in Chamran cultivar and Line9.