Introduction: Nurses, as a large part of the healthcare team, are among the first to manage post-accident injuries. Thus, their readiness to cope with disasters is essential. Objective: This study aimed at assessing the disaster readiness of nurses in hospitals affiliated to Guilan University of Medical Sciences (GUMS) in Rasht City, Iran. Materials and Methods: This across-sectional study was conducted on 177 nurses in hospitals affiliated to GUMS who were selected using a systematic stratified random sampling technique. The data collection tool was Readiness Estimate and Deploy ability Index (READI), i. e., back-translated to Persian, and its acceptable validly and reliability was reported. The obtained data were analyzed using descriptive statistics, including frequency, mean, and standard deviation. Results: The total Mean± SD score of nurses’ disaster readiness was 42± 0. 60. Regarding the dimensions of READI, their Mean± SD scores of disaster readiness in the area of clinical competency, operational competency, survival skills, stress coping, leadership and administrative support, mental attitude, and group integration and identificationwere3. 44± 0. 65, 4. 13± 0. 87, 3. 62± 0. 87, 3. 27± 0. 91, 4± 0. 88, 2. 66± 0. 85, and 3. 82± 0. 85, respectively. The majority of them were moderately ready in all dimensions of disaster readiness, except for the area of operational competency where they were at favorable status. Conclusion: The nurses’ disaster readiness was at a moderate level. Nurses with their technical skills and practical knowledge could provide the highest level of disaster care appropriate to adverse conditions to improve the affected people’ s health. Therefore, improving the nurses’ preparedness level in this field can lead to saving the injured people and reduce the mortality rate.