Background and aims: Non-alcoholic fatty liver disease (NAFLD) is a chronic liver condition that is associated with the changes in transferase enzymes and atherogenic parameters. The aim of this study was to evaluate the effect of eight weeks of high-intensity interval training (HIIT) on plasma levels of liver enzymes and atherogenic markers in NAFLD patients. Methods: In this quasi-experimental study 25 women with NAFLD were randomly selected and allocated to exercise (n=13) and control (n=12) groups. HIIT protocol with an intensity of 80-85% of maximum heart rate was performed for eight weeks, four sessions in a week and 60 minutes in each session (15min warm-up, 30min training exercises, and 15min cooling down). The plasma levels of liver enzymes and atherogenic markers were compared before and after the protocol. Results: After eight weeks of HIIT, in both the intergroup and the intragroup comparisons, a significant increase was detected in plasma levels of HDL-cholesterol. Additionally, in both intergroup and intragroup comparisons, a significant decreases was observed in plasma levels of triglycerides, TC (total cholesterol), LDL-cholesterol, and ALT, as well as LDL/HDL, lipid accumulation product (LAP) index, atherogenic index of plasma (AIP), TC/HDL, body fat percentage, and body mass index (p<0. 05). It should be noted that despite a decrease in the plasma levels of AST compared to the pre-test and control group, it was not statistically significant. Conclusion: According to the findings of this study, HIIT can be suggested as a treatment and prevention strategy for NAFLD.