Growth in the population and increasing the need for food make it necessary to increase agricultural and livestock production; therefore, due to water resources constraints, the optimal use of water in the production process is a requirement of sustainable agriculture and animal husbandry activities. In order to optimize the amount of water used in the production of livestock products, it should be paid attention to the amount of water consumed by the products in the diet ingredient and the amounts of drinking water and water related to livestock maintenance. This amount of water used to produce a livestock production is called virtual water of it. The aim of this study was computing virtual water of Holstein milk production in East Azerbaijan considering three irrigation efficiencies of 33, 60 and 90 percent. Average virtual water of wheat, barley, corn, soybean, beet and alfalfa as a diet element were 1. 2, 1. 5, 0. 13, 3. 8, 2. 2 and 2. 4 cubic meters per a kilogram production of each one by considering 33 percent irrigation efficiency, respectively. According to 33 percent of irrigation efficiency the virtual water of milk for after parturition, high yielding, average yielding and low yielding durations were 1. 5, 1. 4, 2. 4 and 3. 3 cubic meters per a kilogram production, respectively. By increasing irrigation efficiency from 33% to 60% and 90%, the virtual water of milk was decreased by 43 and 60 percent, respectively.