AIMS: One of the effective ways to control weight is to use regular exercise and herbal supplements. The aim of this study was to evaluate the effect of spirulina supplementation, and CRTon plasma levels of resistin, and some indicators of body composition of overweight and obese police officers. MATERIALS & METHODS: The present study was a single-blind, semiexperimental, and applied study. For this purpose, 60 overweight and obese men with a body mass index of more than 25 (kg/m2), and with an age range of 30 to 55 years were purposefully selected, and randomly divided into four groups: training + spirulina, training + placebo, spirulina, and Placebos. The intervention and placebo groups took two 500 mg spirulina, and placebo capsules daily for eight weeks, respectively. CRT were performed for eight weeks, three sessions per week. resistin levels, and some body composition indices were measured before and after exercise. FINDINGS: There was a significant difference in body weight between groups (p<0. 05). In the intragroup study, the values of resistin in the training + spirulina group, body weight, and body mass index in the training + spirulina, training + placebo, and spirulina groups fat percentage, waist-to-hip ratio in the training + spirulina, and training + placebo groups decreased significantly (P<0. 05). CONCLUSION: It seems that spirulina supplementation combined with CRT by reducing the plasma levels of resistin, and some indicators of body composition, can be effective in weight loss, obesity, and cardiovascular health.