This study investigated Macrobenthos density of western white shrimp farms in the Golestan province, Gomishan region. Two saltwater ponds with levels 1 ha and the average depth of 1.5 m was used. In the Gomishan Center and Promote Aquaculture, sampling of sediments from five stations (output, intermediate inputs and sides) was done by using Grab with 262 cm2 surface area during the growing period (from July to October 1389). The samples were stained with Rose Bengal (1 gr/lit) solution later was fixed with 70% ethyl alcohol. Then it was passed through a 500 micron sieve, and the Macrobenthos were remained, which was inspected and counted. Every ten days the temperature, pH and salinity were measured. Macrofauna organisms identified that were related to the category selected in this study were Gastropoda, Bivalvia, polychaete, Insecta, Crustacea. The highest abundances of the ponds 6 and 16 (67% and 58% respectively) are related to Polychaete. Moreover the lowest abundances (2% and 5% respectively) are related to Crustacea. Furthermore, the lowest abundance of Macrobenthos in mentioned ponds belonged to right sides. The highest abundances in ponds of 6 and 16 belonged to input and left sides. The results of T test of the total Macrobenthos abundance comparison between ponds 6 and 16 were insignificant (p>0.05). Macrobenthoses abundance compared in different months (June to September) showed relative decreased, however this difference was significant (p<0.05). Compared factors (temperature, pH and salinity) had not significant differences between the two ponds (p>0.05), however there was significant differences between different months, morning and afternoon (p<0.05).