Introduction: Many educators believe that active participation of clinical nurses in education, is one of the facilitating factors in educational goals. This study aimed to determine obstacles and facilitators of clinical nurse’s cooperation in clinical education of nursing students in Tehran hospitals in 2014.Methods: This is a descriptive analytical study who aimed in Children's Medical Center, Arash, Shariati (Affiliated to Tehran University of Medical Sciences), Rasoul akram, Ali asghar, Akbarabadi (Affiliated to Iran University of Medical Sciences), Taleghani, Modaress and Mofid (Affiliated to Shahid Beheshti University of Medical Sciences) hospitals.384 samples were selected from wards where nursing students were educating clinically. The data collected via a demographic questionnaire. To assess the validity of the questionnaire, content validity was used in quantitative and qualitative terms. In qualitative method, 5 qualified nurses in the field of clinical nursing education as well as 5 faculty members and experts in this regard will be consulted and their corrective comments were taken and included in the questionnaire. In order to assess the reliability of the instruments, a questionnaire in a pilot study was completed in a sample of 20 nurses completed and Cronbach's alpha=0.78 for cooperative barriers questionnaire and a=0.86 for facilitators questionnaire. Data were analyzed by SPSS software version 16 and independent t-test, ANOVA and Tukey test.Results: Nurses status regarding individual obstacles including crowded ward and nurses’ fatigue (57.1%), structural obstacles including weak educational planning (26.5%) and administrative obstacles including patients and their families complain (16.4%), were ranked as the most important obstacles. Nurses having sufficient knowledge and skills (21%), providing a supporting environment and educational planning (57.8%), determine the duties of the students, identifying the person responsible for performance of students (21.2%) were mentioned as the most important facilitators of clinical nurses’ cooperation.Conclusions: Since, one of the difficulties and obstacles relates to individual obstacles, it seems that reducing or overcoming this challenge, can leads to eliminating nurse’s cooperation barriers. This requires collaboration among educators, clinical nurses, educational supervisors, head nurses, nurse managers and other managerial levels, responsible for educational planning and other top managerial levels.