Objective: The objective of the present study was to investigate the effect of combining aqueous extract of saffron and aerobic training on the concentration of hepatic non-enzymatic antioxidants in the STZ-diabetic rats.Methodology: 41 male rats (age: 12 weeks, weight: 314±26.7 gr) were randomly assigned to five groups of healthy-control (HC, n=6), diabetic-control (DC, n=10), diabetic-aerobic training (DAT, n=10), diabetic-saffron (DS, n=10) and diabetic-aerobic training-saffron (DATS, n=5). Except for the healthy-control group, other groups were diabetic by injecting STZ. The diabetic-saffron and diabetic-aerobic training-saffron groups orally received 25 mg/kg aqueous extract of saffron for two weeks per day. The diabetic-aerobic training and diabetic-aerobic training-saffron groups ran on a treadmill for two weeks, five consecutive sessions, with the speed of 12 m/min and slope of %0 for 30 min. Twenty-four hours after the last session of exercise, the rats were anesthetizing by chloroform and sacrificed; hepatic was taken from their bodies and was put in liquid and immediately kept in a deep freezer (-%70) in order to determine the concentration of malondialdehyde (MDA), glutation (GSH) and total antioxidant capacity (TAC).Findings: Analysis of variance (ANOVA) showed significant differences between the five groups at the levels of malondialdehyde, glutation and TAC (P= 0.001). The results of the Scheffe post hoc test demonstrated that the concentration of malondialdehyde had a significant decrease in the saffron-aerobic training (P= 0.001), diabetic-aerobic training (P=0.001) and saffron-diabetic (P=0.001) groups compared with the diabetic-control group. The concentration of glutation had the most and least increase in the saffron-aerobic training and diabetic-control groups, respectively (P= 0.012). The concentration of TAC had the most and least increase in the saffron-aerobic training and diabetic-control groups, respectively (P=0.005).Conclusion: The results of the present study demonstrated that the combination of saffron extract and aerobic training would be appropriate method for reinforcing the hepatic non-enzymatic antioxidant system in diabetic rats.