During 1996, in a potato field at Bojnoord region, some potato plants showing stunting, pale and mild mosaic symptoms were marked. At harvest, a few tubers of each plant were randomly chosen and stored at a dark refrigerator at 4°C until the end of dormancy period Host range, direct double -antibody sandwich enzyme - linked immunosorbent assay (DAS- ELISA), dot immunobinding assay and electron microscopy tests showed that plants which grown from such tubers were infected with Potato Virus X (PVX). Inoculation of indicator plants caused systemic symptoms on Datura stramonium, Lycopersicon esculentum, Nicotiana glutinosa, N.debneyi, N.tabacum cv. Xanthi, N.tabacum cv.Samsun, N.tabacum cv. Turkish and Capsicum frutescent, and local lesion symptoms on Chenopodium amaranticolor, c.quinoa and Gomphrena globosa and did not induce any symptoms on Cucumis satius. The virus was propagated on N.glutinosa for purification and then antiserum was prepared against the purified virus. To determine the distribution of the virus in North of Khorasan, from 26 fields of Bojnoord, Chenaran, Shirvan, Toos, Farooj and Ghochan regions, 247 plants were randomly sampled and DAS -ELISA test was used for detection of PVX. The results showed that the percent of PVX infection in the above regions were 50, 63.6, 34.7, 53.3, 62.8 and 37.2 respectively.