The cercosporoid species, Microcyclospora rumicis Arzanlou & Bakhshi was recently introduced on Rumex crispus L., from Iran (Guilan province, Talesh) (Arzanlou & Bakhshi 2011). Based on the morphological features, this fungus was described as a species of the genus Microcyclospora J. Frank et al. (Arzanlou & Bakhshi 2011). During a phylogenetic study of cercosporoid fungi from north of Iran, the genomic DNA of the type strain (CBS 131546) of the species, was extracted using the protocol developed by Moller et al. (1992). Amplification and sequencing of the internal transcribed spacer regions and intervening 5.8S nrRNA gene of the nrDNA operon (ITS) and partial nuclear ribosomal large subunit (LSU) were done with primers V9G+ITS4 and LR0R+LR5 respectively (Quaedvlieg et al.2013).