KOWSAR MEDICAL JOURNAL
Year:2008 | Volume:13 | Issue:3|Papers Count:14

Objective: In this research the effect of topical application of commercial unboiled honey on open wound healing in male rats were studied.Materials and methods: 50 male rats with weigh of 225±25gr randomly were chosen. Under general anesthesia and sterile condition one round wound with 2cm diameter and full thickness was made on dorsum of neck skin in rats. The day of surgery was considered as zero. Unboiled honey was applied topically on wound of experimental group every two day from time of surgery until appropriate day, control groups wounds were washed with saline. At day three, seven, fourteen and twenty eighth followed surgery rats were killed and samples from wound and adjacent skin were obtained and prepared for routine histological study and stained by H&E and vangisen. Vessels were counted on 3,7 and 14th day and depth of granulation tissue was measured on 7th day. Population of fibroblasts, inflammatory cells and chrome of stained collagen were classified. Tensile strength study was done at 28 day after surgery Data were analyzed by Student-t test.Results: Results showed significant increase in granulation tissues depth (P<0.05). and increase in maximum force for tearing healed wound (P<0.05).angiogenesis was more than control but it wasn’t significant. Density of fibroblasts, emerge of collagen and subsidence of inflammation in experimental group were higher than control.Conclusion: It seems that topical application of honey accelerate wound healing in skin open wound.

Objective: Trichomoniasis is one of the most common parasitic sexually transmitted disease in the world. Different methods are being used for the laboratory diagnosis of trichomoniasis, such as wet mount, various staining methods, Papanicolaou staining, culture, serological techniques and more recently, molecular techniques. In this study, a PCR targeting the 18s rRNA (16S-like ribosomal) gene of T. vaginalis was developed for the detection of the organism.Material and methods: Trichmonas vaginalis isolated from vaginal discharge, then cultivated in Diamond medium culture. DNA of the parasite extracted by phenol chloroform method, then a well-conserved region in the 18s rRNA gene was amplificated successfully.Results: A specific band was detected by PCR giving a single predicted product of 312 bp in gel electrophoresis.Conclusion: Concerning the present result, PCR and designed primers could be recommended for diagnosis of trichomaniasis in the field study.

Objective: Design of a suitable SiRNA against BCR/ABL and using an expressing vector (pRNA-H1.1-Neo) for expression of SiRNA in transfected K562 cell line and evaluate of apoptosis induction value.Matherials and methods: select an suitable sequence from chimeric BCR/ABL mRNA and cloned in expression vector (pRNAH1.1/Neo) as a ShRNA expressing DNA. Then evaluate the cloning by using PCR and sequencing of insert. Cloned vector was transfected to K562 by lipofectamin 2000 protocol and a miss match SiRNA and uncloned vector used as ngative control. The efficacy of transfected method was investigated using GFP expressing vecapoptosis was investigated in transfected cells using cell death detection ELISA kit.Results: The PCR results, electrophoresis and sequencing shown the accuracy of cloned vector and GFP expression with green fleurence confirmed the transfection method with efficacy equal 44%. The apoptosis value in transfected cells with cloned vector in compared with control cells was significantly increased in 24,48 and 72h after transfection. The apoptosis induction was time dependent and 72h after transfection it was 36±3.2% (p<0.0) but in normal ,control vector and negative control cells was respectively, %5.05±0.23, %5.2±0.86 and %4.8±0.6 which was constant with time. Wherase, in cells which transfected with cloned vector, the induction of apoptosis rate was timely and comparable with reduction in BCR/ABL mRNA.Conclusion: The expression pRNA. H1-1/Neo vector was suitably coloned with bcr/abl ShRNA andintracellular SiRNA was expressed which reduced BCR/ABL mRNA and therefore induced apoptosis in K562 cell line. Therefore, specific SiRNA could be attention as a new molecular trapeiutic method for CML.

Background: Previous studies have confirmed the effects of water extract of Crocus sativus on the euphoric and behavioral properties of morphine in male and female mice.Objective: In the present study, the effects of alcohol extract of Crocus sativus stigma on the acquisition and expression of morphine-induced conditioned place preference (CPP) in male Wistar rats (250-300 g) were investigated.Material and Methods: This experimental study was conducted on the 84 male rats that were divided in 18 groups (n=6/group). In a pilot study, different doses of morphine (0.5, 1, 2.5, 5, 7.5 and 10 mg/kg) and the extract (10, 50 and 100 mg/kg) were injected to the animals for evaluation of the drugs ability to induction of place preference. In the second phase of the experiments, the extract of the C. sativus was administered during or after induction of morphine CPP. Then, CPP were tested in the animals. One-way Analysis of Variance (ANOVA) was preformed for statistical procedure. Results: Administration of morphine (0.5, 1, 5, 7.5 and 10 mg/kg), indcreased the time spend in the compartment paired with morphine (i.e. conditioned place preference-CPP). The increment was significant for the dose 7.5 and 10 mg/kg of morphine. Administration of the plant extract (10, 50 and 100 mg/kg) did not change the time spent in the drug-paired side by the animals. Injection of the same doses of the extract before morphine (10 mg/kg) administration caused a decrease in the time spent in drug-paired side in doses 50 and 100 mg/kg of the extract. In addition, injection of the plant extract in the test day to the animals in which reveived morphine (10 mg/kg) in the conditioning days decreased the expression of morphine CPP in the animals which was statisticaly significant for doses 50 and 100 mg/kg of the extract.Conclusion: It could be concluded that injection of the alcoholic extract of C sativus can inhibit the acquisition and expression of morphine-induced CPP in the rats.

Objective: It has been previously documented that hyperoxia pretreatment protects the heart from ischemia-reperfusion injury. The present study was conducted to study the hypothesis that pre-exposure to normobaric hyperoxia reduces cardiomyocyte apoptotic cell death and arrhythmias following induction of ischemia-reperfusion in a rat model of regional coronary artery occlusion.Materials and methods: Male wistar rats pretreated with normal air (21% O2, control, C) and 60 or 180 minutes of hyperoxia (H60 and H180) in hyperoxic chambers (³95% O2), and the hearts were immediately isolated and perfused in a Langendorff apparatus. Hearts were subjected to 30 min of local ischemia followed by 120 min reperfusion. For examining the hyperoxia effects infarct size, percentage of myocytes apoptosis and ischemic arrhythmias were assessed.Results: Infarct size was reduced from 39.5±3.6% in normoxic control group to 26.3±2.3 (p<0.01) and 20.5±2.6 % (p<0.001) in H60 and H180 groups respectively. Apoptosis was decreased by 180 min of hyperoxia (17.3±3.5% in H180 group vs. 31±2.3% in control group, p<0.05). 60 and 180 min of hyperoxia significantly attenuated total number of premature ventricular beats in ischemic phase (p<0.05. Hyperoxia was also reduced VT and VF duration and number of VT episodes (p<0.05). Accordingly, severity of arrhythmias (arrhythmia score) was lower in hyperoxia treated groups compared to control group (p<0.05).Conclusion: The results of present study indicate that hyperoxia pretreatment before heart ischemia and reperfusion reduces infarct size and attenuates cardiomyocyte apoptosis. Hyperoxia also significantly decreases severity of ischemic arrhythmias.

Objective: laryngospasm is a common condition in children after extubation, as it is a critical medical condition, it may threaten patient’s life rapidly And become problematical for Anaesthesiologists. On the other side, intravenous Dexamethasone has many advantageous and carries few complications during anesthesia. In this study we try to research the effects of Dexamethasone on prevention of laryngospasm after extubation in children in comparison with placebo.Matrials and methods: In this double-blind clinical trial, 90 of children who were candidate for elective surgery were chosen randomly and divided to 2 equal groups. Dexamethasone (0.5mg /kg) and placebo were injected intravenously (using coded syringes) to the patient in equal volumes. Results were compared by the use of spss ver 15 software and mann- whitney U test.Results: no laryngospasm seen in the group with dexamethasone injection while 6 people of placebo group experienced laryngospasm after extubation. (14%, P value=0.026).Conclusion: regarding the effect of single dose of Dexamethasone on prevention of subsequent laryngospasm in children during general anesthesia, it is suggested that all children who are going under general anesthesia where it needs endotracheal intubation, should receive single dose of Dexamethasone 0.5mg/kg Iv up to 10mg after induction of anesthesia.

Objective: The aim of this study is evaluating the patterns of antimicrobial resistance acquired urinary tract infections (UTIs) in patients with sepsis in intensive care unit.Materials and methods: One hundred septic patients were hospitalized in a general ICU, besides demographic, clinical, and outcome data were obtained by chart review. Antibiotic resistance/susceptibility was determined using the minimal inhibitory concentration (MIC) technique.Results: A UTI was present in 28 patients that 9 of them were female and 19 of them were male.The mean of ages in the patients was 58.71±19.45 years. Almost all of the patients were resistance to the third generation Cephalosporins.Conclusion: The rate of multidrug-resistant UTIs may be very high in in patients with sepsis admitted in ICU. This antimicrobial susceptibility/ resistance should be determined, and a special antimicrobial treatment protocol should be planned based on the results for each ICU.

Objectives: Dorsalgia is one of the most common diseases in different region of the world. Different factors like sex, age, trunk weight, job and daily activity affect its prevalence. Changes in back spine curvature and forces of its supporting muscles may cause stress on thoracic vertebral column. We studied the relationship between dorsalgia and thoracic spine curvature and its muscle force.Materials and methods: We enrolled 1560 patients from 3 physiotherapy center of Tehran in this analytic cross-sectional study. The patients divided to two groups of patients with complaints of back and shoulder pain and a group of patients (age and height and weight match) with complaint of pain in other part of the body. We used flexible ruler, slope meter, plumb-line to measure the needed values and also examined symmetry of shoulders, back and shoulder muscle force. A 10 question questionnaire was used to measure amount of daily physical activity. Level of significance considered 0.05.Results: Out of 1560 patients, 68.8% (1073) were female and 31.2% (487) were male. No significant difference in age, height and weight of patients in 2 mentioned group was detected (p>0.05). Prevalence of increase in back curvature was 86.9% vs 8.9%, scoliosis 21.2% vs 3.4%, lowpositoned shoulders 34.3% vs 9.6% and lateral deviation of shoulders 23.7% vs 5.12% in first and second group respectively (p<0.05). Regular exercise, paravertebral and shoulder muscle force were also significantly different in two groups (p<0.05).Conclusion: There is a significant relationship between dorsalgia with thoracic spine curvature and its muscle force. General education and self care especially for young peoples and females are necessary to prevent these conditions.

objective: Epidemiological studies have indicated an association between higher body iron stores and coronary artery diseases. It is believed that inflammation and oxidation are important mechanisms involved in the complex and pathological process of atherogenesis. Free radical production is catalyzed and accelerated in the presence of iron that these agents increasing lipid peroxidation special LDL. The determination of plasma ferritin levels is a convenient iron balance assessment method. The purpose of this study was to define the correlation of ferritin levels with severity and extent of coronary artery atherosclerosis.Matherials and methods: In this cross-sectional study that done from April to June 2008 in Shahid Chamran and Sina hospitals in Isfahan, 169 consecutive patients that containing 139 male (82.2%) and 30 female (17.8%) underwent previously indicated coronary artery angiography were selected.Results: Study of Gensini Score (G.S) were revealed that ferritin was significantly correlated with severity and extension of atherosclerosis in the men (p=0.04). Also, there was statistically significant between the diabetes history, FBS>140 mg/dl, mean of systolic and diastolic hypertension and cigarette smoking with G.S (p<0.05). But there no was significant difference between severity and extension of atherosclerosis and ferritin levels with major risk factors such as hypertension, hypercholesterolemia, hypertriglyceridemia, familial coronary diseases history and BMI (p>0.05).Conclusion: Results of this study confirmed a strong correlation of serum ferritin levels with severity and extension of coronary atherosclerosis. However, regarding to the results of this study in men it could have a role in the promotion and augmentation of coronary atherosclerosis, suggestion that ferritin could be a comparable index in patients with ischemic heart disease.

Objective: Quality care of aged patients can effect with vary factors. One of this factors can be nurses attitude tocare of them. The present study was under taken to investigate the attitude about physical care of aged patients.Materials and methods: In this study, attitudes of 100 nurses that employ in internal wards of educational hosoitals inTehran city was determined. This samples selected by random sampling and for data collection used self administered questionnaires.The study was analytical-descriptive and for evaluation of relationship between variables such as age,sex,shift,educational level.job experimental,job satisfaction and attitude of subjects to care of aged patient used inferential and descriptive statistic like mean,sd,chi 2.Z ,T and pearson tests.Results: It was found that nurses attitude to care of aged patients was not appropriate. There were positive significant correlations between age and job satisfaction of samples and their attitudes (p<0.01 and p<0.05).Conclusion: It is recommended that expand planning and education training. Is essential for enhance of nurses attitude and experiments about care of aged patients.