Introduction: The purpose of the present study was to compare the effect of three different types of training programs on serum leptin concentration changes and some regulator hormones of blood glucose in athlete male students.Methods: In this quasi-experimental study, 32 athlete male students were randomly divided into 4 groups: endurance (END), resistance (RES), concurrent (CON) and control (CONT). In an 8-week training, subjects in END group performed 4 sessions per week running on treadmill with 65% heart rate maximum for 45 minutes; RES group received 4 sessions per week consisting of a 9-item circuit resistance of 4 sets with 12 repetitions and intensity of 60% and one repetition maximum. Moreover, CON group performed both of RES and END training protocols. Blood samples were taken before, immediately and 24 hours after last session training. The study results were analyzed using repeated measures Anova (P£0.05).Results: Immediately after exercise, serum leptin concentration decreased significantly in all groups (P£0.05), whereas 24 hours after exercise it increased that was significant in END and RES groups (P£0.001). Cortisol levels increased immediately after exercise though that was not significant. In contrary, it decreased 24 hours after training that was significant just in RES group (P£0.035). Insulin levels decreased, though, not significantly immediately after exercise, whereas it increased 24 hours after exercise significantly in END (P£0.045) and RES groups (P£0.004). Growth hormone also increased immediately and 24 hours after exercise which was not significant in any groups.Conclusion: Serum leptin levels revealed higher decrease in CON group compared to other groups that is probably due to high intensity and long exercise period and more adipose tissue reduction.