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Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources
Title: 
Author(s): 

Issue Info: 
  • Year: 

    0
  • Volume: 

    42
  • Issue: 

    2
  • Pages: 

    -
Measures: 
  • Citations: 

    0
  • Views: 

    1510
  • Downloads: 

    0
Keywords: 
Abstract: 

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Journal: 

Research in Medicine

Issue Info: 
  • Year: 

    2018
  • Volume: 

    42
  • Issue: 

    2
  • Pages: 

    65-70
Measures: 
  • Citations: 

    0
  • Views: 

    1540
  • Downloads: 

    0
Abstract: 

Backgrond: Papillary Thyroid Carcinoma (PTC), unlike other cancers, is completely curable if the treatment begins at early stages of the disease, before any metastasis occurs. One of the common places that the cancer may invade is the neck lymph nodes. On this basis, many surgeons suggest prophylactic central neck lymph nodes dissection in such patients, but others do not agree because of the risks that may exceed the benefits of this approach. Despite the presence of several studies in this regard, the debate is still ongoing. Therefore, the present study was conducted to examine the effect of prophylactic central neck lymph node dissection on preventing recurrence in patients with PTC.Materials & Methods: In the current clinical trial, a total of 122 patients with PTC were enrolled who were categorized in two equal 61-patient groups, with (case) and without (control) prophylactic central neck lymph nodes dissection. Short-term (up to 10 days post-operation) and long-term (six months post-operation) consequences were compared between the two groups. None of the patients had evidences of central cervical lymph node involvement before operation. The results were analyzed using Chi-square and t-test.Results: Both groups were comparable in terms of age (mean, 46.1 years in the case group and 45.8 years in the control group) and sex (10 males in the case group and 11 males in the control group). In the case group, there were 16 patients (26.2%) with pathology-proven central cervical lymph node involvement. Serum calcium level dropped more significantly in the case group immediately after the surgery, but returned to a comparable level with that in the controls at the six-month time-point. Comparison of cases and controls showed no significant difference in terms of vocal cord involvement (4.9% vs.3.3%, respectively; p=0.50), recurrent mass (0% vs.3%, respectively; p=0.49) or new regional lymphadenopathy (1.6% vs.7%, respectively; p=0.37). None of the patients in the study deceased nor had recurrent nerve injury.Conclusion: Prophylactic central cervical lymph node dissection has no significant effect on the occurrence rate of short-term/long-term complications of surgery in patients with PTC.

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Journal: 

Research in Medicine

Issue Info: 
  • Year: 

    2018
  • Volume: 

    42
  • Issue: 

    2
  • Pages: 

    71-78
Measures: 
  • Citations: 

    0
  • Views: 

    1039
  • Downloads: 

    0
Abstract: 

Background: Considering the importance of rapid detection of cholera toxin in environmental samples, an immunoassay method was developed for direct detection of cytotoxin B.Materials and Methods: The gene sequence of ctxB was cloned to pET28a vector and the recombinant protein was expressed in BL21 (DE3). The recombinant CtxB was purified via affinity chromatography and then used for immunization of chickens. The polyclonal anti-CtxB antibodies were purified from egg yolks using polyethylene glycol. The IgY antibodies were used to develop a sandwich ELISA. Also, analytical sensitivity of the assay was determined.Results: A total of 38 mg of recombinant CtxB, more than %95 purity, was purified from 1 liter of bacterial culture. The titer of anti-CtxB antibodies in the serum sample of immunized chicken was 1: 32, 000. The yield of purified IgY was 50.9 mg per egg yolk. The detection limit of the developed ELISA was about 33 pg/ml of recombinant CtxB.Conclusion: Management and control of Vibrio cholerae infection requires the availability of rapid diagnostic tools. The newly developed immunoassay provides a rapid and sensitive method for the detection of cytotoxin B of V. cholerae. We plan to evaluate the performance of the developed assay in the detection of cholera toxin in environmental and food samples in the following studies.

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Journal: 

Research in Medicine

Issue Info: 
  • Year: 

    2018
  • Volume: 

    42
  • Issue: 

    2
  • Pages: 

    79-85
Measures: 
  • Citations: 

    0
  • Views: 

    789
  • Downloads: 

    0
Abstract: 

Background: The material is always covered by proteins immediately upon contact with a physiological environment. Hemoglobin (Hb) is one of the major blood proteins. Due to the entry of substances into blood, hemoglobin may interact with them. The interaction between Hemoglobin and ligands such as nanoparticles is very important and may cause structural and functional changes on Hemoglobin. According to the recent studies, PEG (poly ethylene glycol) coated nanoparticles have better biocompatibility compared with noncoated nanoparticles, thus the present study was conducted to investigate the interaction of PEG-silver nanoparticles with hemoglobin.Materials and Methods: In the present experimental study, various spectroscopic methods, including UV Visible, fluorescence, and Circular Dichroism (CD), were used to evaluate the interaction of silver nanoparticles with polyethylene glycol coating and hemoglobin. Using these methods, the effects of nanoparticles on the second structure and variations in the alpha helix and beta sheets of the protein, as well as changes in the third structure, and thus the change in the pr otein function, were investigated.Results: The results of fluorescence spectroscopy and UV/Vis absorption spectroscopy indicated that the structure of the Trp residue environments was altered and the physiological functions of Hb were affected by silver nanoparticles coated with poly ethylene glycol. It could also be suggested that due to the binding of silver nanoparticle to Hb, some conformational changes are induced, enhancing the hydrophobicity.These nanoparticles have increased the ratio of deoxy and decreased hemoglobin tendency to oxygen. This increase in the highest concentration of nanoparticles is six times as common.Conclusion: The results showed that PEGylated silver nanoparticles lead to secondary structural changes and hemoglobin unfolding. Therefore, PEGylated silver nanoparticles decrease the hemoglobin function by decreasing the hemoglobin tendency toward oxygen. As a result, silver nanoparticles may cause the molecular toxicity of proteins. Considering the results obtained and the importance of nanoparticles biosafety, it is suggested that by changing the coating, size, or shape of nanoparticles and optimizing them, the possible side effects of nanoparticles on the structur e and function of the protein can be prevented.

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Journal: 

Research in Medicine

Issue Info: 
  • Year: 

    2018
  • Volume: 

    42
  • Issue: 

    2
  • Pages: 

    86-92
Measures: 
  • Citations: 

    0
  • Views: 

    636
  • Downloads: 

    0
Abstract: 

Backgroundm: Betatrophin is known as a new therapeutic target of pancreases tissue. The aim of the present study was to investigate the effect of endurance training program on betatrophin serum concentrations and pathological changes of pancreas tissue in diabetic rats.Materials and Methods: In the present experimental study, male wistar rats (N=24) were divided into three groups of healthy control, diabetic control, and endurance training. After induction of diabetes, the training group performed four weeks of endurance training and betatrophin concentrations were assessed using ELIZA. Also, the pancreatic tissue was evaluated using histological methods. Data were analyzed using one way ANOVA.Results: The results showed that betatrophin serum concentration significantly decreased in diabetic control group (P£0.05), but no significant increase was observed in the training group (P³0.05). Compared with diabetic control groups, endurance training did not make any significant changes in insulin resistance and beta cell function (P³0.05). In endurance training group, the means of number and size of pancreatic islets and the number of b-cell were higher than those of diabetic control group.Conclusion: Although endurance training did not significantly increase the betatrophin levels, it had a positive effect on the pancreatic tissue in diabetic rats.

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Journal: 

Research in Medicine

Issue Info: 
  • Year: 

    2018
  • Volume: 

    42
  • Issue: 

    2
  • Pages: 

    93-99
Measures: 
  • Citations: 

    0
  • Views: 

    684
  • Downloads: 

    0
Abstract: 

Background: Magnesium (Mg2+) is the second most abundant cation (after potassium) in the cell and plays an important role in various biological functions, including cell cycle, channel regulation, ATPase activity, metabolic regulation, etc. Magnesium deficiency increased incidence of cardiovascular diseases, including hypertension, stroke, and atherosclerosis as well as gastrointestinal disorders, such as loss of appetite, nausea, and vomiting. Considering the antioxidant activity of magnesium and the role of oxidative stress in the pathophysiology of epileptic seizures, the present study was conducted to investigate the effect of magnesium sulfate on pentylenetetrazole (PTZ) -induced seizures in adult male Wistar rats.Methods: In the present experimental study, the rats were randomly divided into eight groups: normal control, magnesium sulfate (0.05, 0.1, and 0.2 g/kg intragastrically, daily) alone, seizuric control rats (PTZ, 35 mg/ kg, i.p.), magnesium sulfate (0.05, 0.1, and 0.2 g/kg intragastrically, daily) together with PTZ, and treatment was performed accordingly. Administration of magnesium sulfate (0.05, 0.1 and 0.2 g/kg) was launched 1.5 h before the first dose of PTZ and continued up to 28 days. The rats were sacrificed on day 29 and parameters of oxidative stress, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), and malondialdehyde (MDA) activity were measured in liver homogenate. Data were evaluated running oneway analysis of variance.Results: Administration of magnesium sulfate (0.1 and 0.2 g/kg) significantly increased the levels of antioxidant enzymes, including SOD, CAT, GPX, while it decreased MDA levels (p<0.05).Conclusion: It seems that presumably magnesium sulfate is effective in providing protection against oxidative stress induced by PTZ.

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Journal: 

Research in Medicine

Issue Info: 
  • Year: 

    2018
  • Volume: 

    42
  • Issue: 

    2
  • Pages: 

    100-105
Measures: 
  • Citations: 

    1
  • Views: 

    967
  • Downloads: 

    0
Abstract: 

Background: Self-efficacy is one of the most important psychological constructs in numerous health areas, including reproductive health. It is necessary to use an appropriate tool for measuring selfefficacy in people coping with challenge of infertility treatment. The purpose of the present study was to determine the psychometric properties of infertility self-efficacy scale (ISE) in Iranian infertile women.Materials and Methods: The present descriptive study was conducted at Mehr infertility clinic in Rasht in summer 2017. A total of 203 infertile women who were referred to a private infertility clinic were selected using available sampling. They responded to ISE, Beck Depression inventory (BDI-2), Infertility stress questionnaire (FPI), and stress coping strategies questionnaire (CISS). To assess the reliability, internal consistency was calculated and cronbach’s alpha was obtained. Also, for the purpose of convergent validity, Stress Coping Strategies questionnaire was administered and to investigate the divergent validity, we used Infertility stress questionnaire and Beck Depression Inventory. Moreover, to assess construct validity of the ISE, exploratory factor analysis with principal component was implemented.Results: The Cronbach’s coefficient for the scale was 0.90, which shows an excellent internal consistency.Also, the results of exploratory factor analysis supported one component for the ISE, which was similar to that of the original scale. Convergent validity of the ISE with Stress coping strategies questionnaire was calculated and statistically significant correlation was obtained. The divergent validity of the ISE revealed significant negative correlation between ISE and Beck depression Inventory and Infertility stress questionnaire.Conclusion: It seems that the ISE is a reliable and valid measure to assess self-efficacy among a sample of Iranian women who underwent infertility treatment. The ISE can be used as a useful self-assessment tool for clinical researches and psychological counseling.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: 

Research in Medicine

Issue Info: 
  • Year: 

    2018
  • Volume: 

    42
  • Issue: 

    2
  • Pages: 

    106-109
Measures: 
  • Citations: 

    0
  • Views: 

    785
  • Downloads: 

    0
Abstract: 

Background: The previous studies suggest that Retinal Nerve Fiber Layer (RNFL) thickness decreases over time in Multiple Sclerosis (MS) and can be a marker of disease activity for patients’ follow-up. Here, we aimed to compare RNFL thickness in patients with Relapsing Remitting MS (RRMS) during three years of follow-up.Methods: In the present prospective study, patients with diagnosis of RRMS and history of optic neuritis were included.Demographic features and disease characteristics were collected and underwent Optical Coherence Tomography (OCT). OCT was repeated after three years and the obtained values were compared.Results: A total of 12 patients (11 women) with the mean age of 35.6 ± 6.1 years and mean disease duration of 5.3 ± 5.8 were included in the study. RNFL thicknesses were 101.27 ± 9.15 mm and 92.00 ±7.96 mm in the left eye at the baseline and after 3 years, respectively (P-value<0.001). In the right eye, RNFL thicknesses were 99.72 ± 16.37 mm and 86.36 ± 20.35 mm at the baseline and after 3 years, respectively (p=0.014).Conclusions: We found a significant decrease in RNFL thickness in RRMS patients after 3 years of follow up, in spite of getting immunomodulatory treatment. We recommend OCT as an inexpensive and feasible tool for outcome measurement in RRMS patients.

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Journal: 

Research in Medicine

Issue Info: 
  • Year: 

    2018
  • Volume: 

    42
  • Issue: 

    2
  • Pages: 

    110-115
Measures: 
  • Citations: 

    0
  • Views: 

    637
  • Downloads: 

    0
Abstract: 

Background: Acute Myeloid Leukemia (AML) is the second most prevalent blood cancer in Iran and the fifth prevalent cancer in the world, which involves 8% of all the cancers. MicroRNAs are a class of small non-coding RNAs which have recently been shown to play a crucial role in major cellular processes such as development and differentiation, which can change the genes regulation that may lead to cancer development.So far, the miR-199b expression has been studied in many types of solid cancers; therefore, the purpose of the present study was to determine the miR-199b expression in AML. type of blood cancer.Materials and Methods: In the present case-control study, miR199b expression was investigated in 30 patients with AML admitted to Mirzakochak khan Jangali Hospital, in 2016, using Real time PCR and the results were compared with those of the same number of healthy individuals as control. Statistical analyses were performed using SPSS (version 19), Graphpad, and Prism 6 running t-tests. P values<0.05 were considered as significant.Results: There was a statistically significant reduction in miR199b expression in patients as compared with control group (P=0.0001) while no correlation was observed between patients’ age (P=0.545) and sex (P=0.704) with miR-199b down-regulation among the studied population.Conclusion: Mir-199b, as a tumor suppressor, showed a reduction in its expression. Therefore, evaluation of expression of that can be used as a diagnostic agent and also a prognostic factor in patients with AML.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: 

Research in Medicine

Issue Info: 
  • Year: 

    2018
  • Volume: 

    42
  • Issue: 

    2
  • Pages: 

    116-120
Measures: 
  • Citations: 

    0
  • Views: 

    723
  • Downloads: 

    0
Abstract: 

Background: Congenital Adrenal Hyperplasia (CAH) is an inherited hereditary autosomal recessive heredity, which is often induced by mutation in the CYP21A2 gene. The aim of the present study was to determine the prevalence of cluster exon 6 mutation among patients with CAH deficient in 21-OHD enzyme in Iranian population.Materials and Methods: In the current descriptive study, blood samples were collected from 25 patients with CAH who referred to Ali Asghar Hospital in Tehran. After extraction of genomic DNA, the region containing the cluster gene exon 6 gene was amplified using PCR and then sequenced. Finally, by analyzing sequences, the frequency of cluster mutation exon 6 was determined in the population studied.Results: A total of 15 patients (60%) had a cluster mutation of exon 6, so that in all of them, three mutations V236E, I236N, and M238K were observed as heterozygote. This mutation in the healthy parents of these patients was also identified as heterozygote.Conclusion: It seems that cluster E6 mutation in the heterozygote form alone does not lead to disease, and it is necessary to examine the presence of other mutations in the gene. This mutation may be present as compound heterozygote along with other mutations in the rest of the exons in patients.

Yearly Impact: مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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