QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL
Year:2020 | Volume:13 | Issue:12|Papers Count:8

Background and Objectives: Spermatogonial stem cells are specific cells that have the ability of self-renewal and differentiation. These cells play an essential role in maintaining spermatogenesis and fertility. In this regard, the present study was performed with the purpose of investigating the effect of different concentrations of follicle stimulating hormone (FSH) on in vitro colony formation of caprine spermatogonial stem cells. Methods: Spermatogonial cells, were isolated from prepubertal goat testis using two-step enzymatic digestion. Then, isolated cells were cultured for 10 days in four groups. In the control group, simple culture of spermatogonial cells was performed in Dulbecco's Modified Eagle Medium (DMEM) containing 1% antibiotics and 5% FBS (Fetal Bovine Serum). In the treatment groups 1, 2, and 3, different concentrations of follicle stimulating hormone (5, 10, and 20 IU/ml), was added to the culture medium, respectively. The culture media were changed every 72 hours. Identification of cells was confirmed by immunocytochemical staining against PGP9. 5 antigen. Immediately after isolation, percentage of cells viability, surface area, and number of colonies formed on 4th, 7th and 10th days after the culture, were evaluated using aninverted microscope. Data were analyzed using one way ANOVA test. Results: The findings indicated that viability rate of Spermatogonial stem cells after isolation was 89. 4 ± 2. 32%. The effect of FSH on the formation of spermatogonial cells colonies was dose dependent. Doses of 5 and 10 IU/ml increased the surface area and number of the spermatogonial cell derived colonies but dose of 20 IU/ml reduced colonies formation (p < 0. 05). Conclusion: FSH can provide an appropriate culture medium for the study of spermatogonial cells in vitro.

Background and Objectives: Previouse studies demonstrated inverse association between healthy eating index and alot of chronic diseases like cardiovascular disease and cancer and mortality. Since there are limited study about relation between healthy eating index and reting metabolic rate. considering the results of some studies about the relation between weight, body composition and blood suger and considering the association between these factores and RMR, the aim of this study is to investigate this association. Methods: this study is a cross sectional study that was conducted on 256 subjects who were above 18 years old. Body composition, resting metabolic rate, were evaluated. Dietary intakes including macronutrient and energy intake of participant were mentioned using a validated food frequency questionnaire 168 items. resting metabolic rate was evaluated by inderect calorymetry(MetaLyzer 3B-R3) and body composition was assested by Multi-frequency bioelectrical impedance analyzer InBody 770 scanner (InBody co, Seoul, Korea). To evalute relation between healthy eating index with body composition and resting metabolic rate, ANCOVA was used. Also curve estimation was performed to find linear association between RMR and HEI. Result: this study did not found significant relation between healthy eating index and resting metabolic rate in both gender(men: p=0. 83, women: p= 0. 9). The score of healthy eating index in men was higher than women and with age increased, the score of healthy eating index increased. Conclusion: Current study did not demostrate any significant differences between resting metabolic rate and body composition in tertiles of healthy eating index.

Background and Objectives: Epilepsy is a neurological disorder caused by abnormal electrical discharges from the brain. Carbamazepine is used to treat epilepsy. Over time, this drug may increase oxidative stress and reproductive abnormalities in patients with epilepsy. Wheat germ oil has the highest amount of vitamin E, among other vegetable oils. The aim of this study was to evaluate the effect of wheat germ oil as an antioxidant-rich source on oxidative stress and in vitro fertilization in carbamazepine-treated epileptic male mice. Methods: In this experimental study, 40 adult maleand 20 adult female Balb/C strain mice with a mean weight of 25-30g were used. Male animals divided into 4 groups (n=10), including: control; pentylenetetrazole (40 mg/kg/day); pentylenetetrazole (40 mg/kg/day) + carbamazepine (180 mg/kg/day) andpentylenetetrazole (40 mg/kg/day) +carbamazepine (180 mg/kg/day) + wheat germ oil (500 mg/kg/day). All doses were given to the male rats orally for 42 days based on g/kg body weight. Five healthy female mice were used for ovulation in each group. At the end of the treatment period, quantity of catalase enzyme, total antioxidant capacity, fertilization, zygote development, were examined. Data were analyzed using one-way ANOVA in SPSS19 software. Results: The results of this study showed that administration of wheat germ oil in the carbamazepine-treated group significantly (P <0. 05) increased amount of the catalase, total antioxidant capacity, fertilization and zygote development in the laboratory. Conclusion: Wheat germ oil acts as an antioxidant agent and increases the amount of catalase, total antioxidant capacity and in vitro fertilization by reducing free radicals due to epilepsy and carbamazepine consumption.

Background and Objectives: Intense exercise results in the production of free radicals and damage caused by oxidative stress. In this regard, the present study aimed to investigate the effect of Silybummarianum consumption along with a period of increasing exercises on the oxidative response to an acute exercise session in young wrestlers. Methods: A total of 20 well-exercised wrestlers, were randomly divided into two groups of exercise along with Silybummarianum consumption and exercise along with placebo consumption after obtaining written consent. The Silybummarianum supplement group was supplemented at a dose of 300 mg, three times a day for four weeks. Blood samples were taken before and immediately after an equal training session before supplementation and after one month of supplementation. In order to analyze the data, repeated measures analysis of variance and independent t-test were used. Results: In terms of between group differences, there was a significant increase in superoxide dismutase levels before exercising and after supplementation (p = 0. 032) and immediately after exercise and after supplementation (p = 0. 002) in the Silybummarianum group. There was also a significant decrease in malondialdehyde levels before exercise and after supplementation (p = 0. 001) and immediately after supplementation (p = 0. 001) in the Silybummarianum group. Moreover, there was a significant increase in total antioxidant values before exercise after supplementation (p = 0. 001) and immediately after exercise and after supplementation (p = 0. 008) in the Silybummarianum group. According to the repeated measures ANOVA, the mean level of malondialdehyde in the Silybummarianum group was lower than the control group. Conclusion: Therefore, it can be concluded that the consumption of Silybummarianum in increasing exercises leads to the decrease of oxidant indexes and increase in antioxidant indexes.

Background and Objectives: Varicocele is one of the most common causes of male infertility. Varicocele is an abnormal dilatation and tortuosity of veins of the pampiniform plexus, which drain the testis. Studies have shown that elevated level of oxidative stress markers, such as nitric oxide (NO) in the dilated veins of patients with varicocele impair testicular function. The aim of this study, was to investigate the relationship between nitric oxide synthase 3 (NOS3), T-786C, and 4a4b gene polymorphism as a common genetic factor with the risk of varicocele in Iranian men. Methods: The association of NOS3 T-786C and 4a4b gene polymorphisms in 60 Iranian men with varicocele and 61 control samples, were investigated using Multiplex-ARMS PCR and conventional PCR techniques. Data were statistically analyzed by t-test at the significance level of p<0. 05. Results: The results revealed that among 60men with varicocele, 95% had-786 TTgenotype, 3. 3% had heterozygotic genotype T-786C, and 1. 6%, were CC in T-786C polymorphism. In addition, just 5% were heterozygote (ab) in 4a4b polymorphism and 95% had wild type genotype (aa), which was not statistically significant. Conclusion: In this study, the majority of individuals had wild-type genotypes TT and aa in T-786C and 4a4b polymorphisms, respectively. According to this data, no significant differences were found between NOS3 gene T-786C and 4a4b polymorphisms in the individuals with varicocele. It is worth noting that further studies should be performed to confirm these findings.

Background and Objectives: Pseudomonas aeruginosastrains isolated from lungs of cystic fibrosis (CF) patients are often heterogeneous and antibiotic resistant strains. In this regard, in the current study, the antibiotic resistance properties and genetic diversity of these bacteria, were investigated using repetitive-element-based molecular assay in the samples isolated from patients in Iran. Methods: This study was performed as a descriptive cross-sectional study on 100 strains of P. aeruginosa isolated from CF patients. The isolates were diagnosed using standard biochemical tests and then their antibiotic resistance pattern was determined. Molecular diversity of the strains was determined by Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction (ERIC-PCR) and BOX A1R-PCR methods. The correlation between molecular types and antibiotic resistance pattern, was determined by chisquare test. Results: The findings indicated that the prevalence of multiple drug resistant isolates was 35%, while, only two strains hadhypermutator phenotypes (HP). Most of the isolates (96%), were resistant to rifampin and the highest susceptibility, was to streptomycin, imipenem, and meropenem (96%, 93%, and 94%, respectively). Molecular analysis revealed that BOXA1R-PCR fingerprinting produced 24 patterns in 8 clusters, while, ERIC-PCR resulted in 26 patterns in 9 clusters in the total population. Conclusion: The high prevalence of diversity and multiple antibiotic resistance in P. aeruginosa strains isolated fromCF patientsthreatens the public health in Iran. Therefore, the findings of the present studycould lead to understanding of the evolution of this bacterium in CF patients and help to find newer drug targets for the control of chronic CF infection.

Background and Objectives: The present experimental study was aimed to investigate the effect of T. dioica flower hydroalcoholic extract on thioacetamide induced histopathological changes. Methods: Thirty adult male Wistar rats, were randomly divided into three groups of control (normal saline); negative control (thioacetamide 50 mg/kg) and group that received thioacetamide and T. dioica flower extract (200 mg/kg). The third group received the T. dioica flowers extract two weeks before the injection of thioacetamide and two weeks after the injection of thioacetamide. The healthy control group and the negative control group received normal saline. At the end of the experiment, blood samples, were obtained from the heart to determine lever enzymes aspartate aminotransferase (AST), alanine amino transaminase (ALT), blood urea nitrogen (BUN), and creatinine. After euthanasia, tissue samples, were examined using a light microscope after staining with haematoxylin-eosin. Data were analyzed by one-way ANOVA statistical test. Statistical significance was considered as p < 0. 05. Results: The levels of serum liver enzyme, were significantly increased after the administration of thioacetamide. The administration of T. dioica extract significantly decreased serum ALT and AST levels compared to the negative control group (p < 0. 05). Moreover, treatment with T. dioica extract significantly reduced serum BUN and creatinine levels compared to the negative control group (p < 0. 05). In the histomorphometric investigation of liver, the fatty change and necrosis was lower in the T. dioica – treated group compared to the negative control group (p < 0. 05). Renal histopathological examination also revealed lower kidney damage compared to the group treated with thioacetamide. Conclusion: hydro-alcoholic extract of T. dioica flowers can reduce thioacetamide induced liver and kidney injury.

Background and Objectives: Effective relationship between teacher and student is one of the most important indicators of effective teaching and a strong stimulus for learning. The present research was conducted with the objective of designing validating the questionnaire of factors affecting teacher-student relationship from the viewpoint of students of Qom University of Medical Sciences in 2015. Methods: In this exploratory study, the questionnaire of the relationship between teachers and students, was designed with the initial list of 46 questions and the participation of 20 students and 5 faculty members. Questionnaire Validation was performed using face validity and content validity and determination of the construct validity, was carried out using exploratory and confirmatory factor analysis. Cronbach alpha coefficient and intra-class correlation coefficient tests were used to determine the reliability of the questionnaire. Data were analyzed by exploratory and confirmatory factor analysis and using SPSS version 16 and Lisrel 8. 80 soft wares. Results: The results showed that 35 questions of the Teacher-Student Relationship questionnaire had an acceptable content validity (0. 68 to 0. 97). Measures of the Cronbach’ s alpha coefficient and intra-class correlation coefficient, were reported to be 0. 90 and 0. 85, respectively. Exploratory factor analysis reduced the questions to 22 questions and categorized the items to five factors (individual ability, academic ability, communication, professional ethics, religion, and belief, and overall, explained a total of 54% of all variance of these variables. Conclusion: The findings of this research indicated that the factors affecting the relationship between teachers and students, have acceptable validity and reliability and can be used in research activities.