In this investigation, we have reportedthe synthesis of the complex [Th(5, 5´-dmbpy)3](NO3)4](1)that 5, 5´-dmbpy =5, 5´-dimethyl-2, 2´-bipyridine. This complex has been characterized using spectral methods (FT-IR, UV-Vis, 1H-NMR and luminescence), elemental analysis and the Cyclic Voltammetry (CV) method. The FT-IR data shows that 5, 5´-dimethyl-2, 2´-bipyridine (5, 5´-dmbpy) ligand adduct to metal centers by nitrogen atoms. Electronic spectra of (1) shows ligand field transitions and charge transfer (CT) bands. Electrochemical data for the complex in the DMF solution show reduction and oxidationprocessesfor the Thorium ionandligand. The interactions of complex (1)withFS-DNAhave been investigated using UV– Vis and gel electrophoresis. The binding constant (Kb) was calculated using UV– Vis technique (Kb=3. 5×105). Also, DNA cleavage was studied using agarose gel electrophoresis. The antibacterial properties and fluorescence property of complex (1) have also been examined. The in vitro biological screening effects of the complex and ligand were tested against different types of bacterium and [Th(5, 5´-dmbpy)3](NO3)4](1)shows antibacterial properties.