Iranian Scientific Fisheries Journal
Year:2016 | Volume:25 | Issue:5|Papers Count:21

The aim of this study was to determine the concentration of iron and Hazard quotient (HQ) in muscle of four marine fishes, orange - spotted grouper(Epinephelus coioides),yellow fin sea bream (Acanthopagrus latus), tongue fishes (Cynoglossus arel) ,silver pomfret (Pampus argenteus) and farmed carp fish, common carp (Cyprinus carpio), grass carp (Ctenopharyngodon idella), big head carp (Aristichthys nobilis) and silver carp (Hypophthalmichthys molitrix) from Khuzestan. Farmed fish sample was prepared from the Azadegan warm water fish culture center and marine fish was prepared from fishing ports Bahrkan, Abadan and Bandar Emam. Iron concentration measured by wet digestion and atomic absorption spectrometer Perkin Elmer 4100.The results showed the average of iron level in farmed fish was 17.08±1.21 mg/kg dry weight and in marine fish was 10.68±3.66 mg/kg dry weight (P<0.05).The results showed that the highest iron concentrations between farmed fish muscle was on big head carp equal to 18.16mg/kg dry weight and was significantly different (P <0.05) with grass carp and the highest iron concentrations between marine fish muscle was onTongue fishequal to15.50±0.50 mg/kg dry weight and was significantly different (P <0.05). Average hazard quotient in farmed fish was HQ=0.61and in marine fish was HQ =0.38. Hazard quotientin marine fish were in tongue fish HQ=0.55, yellow fin sea bream HQ =0. 3, Orange -spotted grouper HQ=0.26 and silver pomfret HQ =0.41 and Hazard quotient in fish were in big head HQ=0.65, common carp HQ =0.63 grass carp HQ=0.55and silver carp HQ =0.61. Hazard quotient in all samples was less than one and so fish nutrition no risk in terms of the amount of iron in the human body.

The relationship between diversity or density of phytoplankton and other marine fisheries sector is of great importance. The study of Spatial and temporal variability of distribution of phytoplankton communities in the coastal waters of the Northern Persian Gulf (QESHM, LENGEH) were studied during 2008. In this study, five groups (47 genera) of phytoplankton include, Bacillariophyceae, Dinophyceae, Cyanophyceae, Crysophyceae and Euglenophyceae were identified. The result showed that Diatoms group (79.6 %) and Rhizosolenia genus (45. 5 %), contributed more towards the percentage composition of different groups and genera of phytoplankton at the during Study. The annual changes in relative abundance and Margalef, Shannon, pielu and Simpson indices were estimated, 0.58-2.56, 0.08-0.97, 0.23-2.6, 0.09-0.93 respectively. Multivariate analysis of variance showed that during the study, density and biological indicators of phytoplankton were significant between seasons (p<0.05). The spatial cluster analysis based on similarity coefficients obtained two clusters in the areas studied. According to the results obtained can be concluded that both the area of ecological conditions in ranks are instead moderate. Also, phytoplankton of diversity and richness is good.

This research in line with fish cage culture plan in the southern coast of the Caspian Sea was carried out. Sampling was seasonally in 8 transects at the depth of 5-100 m from Astara to Torkaman areas in 2008-2009. The aim of this study was to investigate the distribution, abundance and biomass of macrobenthic invertebrate. The highest abundance and biomass were found in transects of Amirabad with average 10931.7±7301.4N/m2 and Astara with mean 86.2±160.3g/m2, respectively. This could be due to the presence of alien species into the Caspian Sea, Streblospio gynobranchiata in 2003 and is the dominant population of macrobenthic invertebrate concern up to 58.4% and increased of S. gynobranchiata (high power compatibility) and mussels of Cerastoderma glaucum (large size) is relevant, respectively. The abundance and biomass have fluctuations in different seasons and the highest abundance with average 6280.1±5693.1N/m2 and biomass with mean 66.1±126.9g/m2 was in winter and spring, respectively. A significant difference between abundance and transects (p<0.05), no significant difference between abundance and depth and seasons (p>0.05), significant difference between biomass and depth (p<0.05) and no significant difference between biomass at transect and seasons (p>0.05) were found. Also, according to multivariate analysis of benthic invertebrate on the abundance at various depths revealed that depths of 5, 10 and 20 meters from the importance of certain fisheries in terms of frequency, abundance and biomass of the macrobenthic invertebrate, slope substrate, substrate, close to the beach, the accumulation of nutrients, Nursery ground, feeding ground and Spawning ground is important for different important species in the Caspian Sea. Therefore, the establishment of fish cage culture was recommended more than 20-meter depths according to the plans of fish cage culture in the southern coast of the Caspian Sea of the Iranian Fisheries Organization.

Aeromonas are an example of emerging bacterial pathogens. Even though they have been recognized as primary fish and human pathogens. Aeromonas hydrophila are opportunistic pathogens that are at the same time infectious and enterotoxigenic and multiple antibiotic resistances (MAR) among Aeromonas hydrophila strains has been reported from many parts of the world. Under these circumstances, it will be worthwhile to find out the prevalence of antibiotic resistance of the Aeromonas hydrophila strains. The one hundred pieces of fish samples were collected from 4 common carp training pool in Khuzestan province. The part of intestine was collected in sterile plate and was homogenized. The samples were cultured in blood agar and incubated in 37centigrade degree temperature. Three to five Aeomonas hydrophila suspected colony, were selected from any plate and purified in blood agar. After initial evaluation of each colony by catalase, oxidase and gram staining, suspected strains DNA was extracted by boiling. Aeromonas hydrophila strains were confirmed by PCR assay and using of genus and species specific primers. Finally, multiple antibiotic resistance (MAR) of confirmed Aeromonas hydrophila isolates was evaluated by Kirby-Bauer disc diffusion method. Twenty Aeromonas hydrophila strains of Aeromonas suspected colonies were confirmed by PCR assay and the most of isolates had a multiple resistance. The least and the most resistance was observed regarding to cefotaxim and ciprofloxacin (<25%), vancomycin and clindamycin (90%), respectively. Compared with results of other studies, antibiotic resistance pattern of these bacterial strains is variable in different geographical areas; therefore resistant pattern of each group of bacteria must be determined in each area.

Biological and ecological studies of water resources play an important role in both researches and scientific studies. The most important step for this study is identifying the characteristics of the ecosystem, living organisms and environmental factors. In recent years, Water quality evaluation, especially drinkable water, became both important and essential for reduction in quality and quantity of them. Lakes and ponds always being under attention as the most important resources for drinking and agricultural purposes. To improve efficiency of uses of the water resources, we need appropriate methods to determine quality of them. Investigate the quality of the Zayandeh roud lake water as an important resource to supplying drinking and agricultural water, in its basin and other province is necessary. In this study after determining 5 stations in different parts of the lake, sampling was done one time per every 45 days. WQI index calculated with use of 6 parameters: No2, No3, DO, EC, Hardness and, pH. However, despite of decreasing of the water quality of zayandeh roud lake, but according to WQI index, water of the lake was in the good category (50-100). This study was also showed that the water quality of zayandeh roud lake need a comprehensive management.

Sexual dimorphism is a phenomenon among some fish species such as the members of the genus Aphanius. Among species with sexual dimorphism, morphological analysis needs to be performed separately. Hence, this investigation was carried out to determine suitable sex of Aphanius dispar for morphological studies. A total 180 specimens (male and female) were collected from populations of Sarbaz, Mehran and Shour rivers. Digital picture of speciments were taken from their left sides and then 17 landmark points cover their body shape were determined and digitized using tpsDig2 software. The data after GPA, were analyzed using PCA, CVA and MANOVA. The results revealed that studied population can be differitiated based on their body shap but between male and female of the Shour and Mehran rivers did not found significant different ib their separate analysis. Sexual dimorphism in the head and trunk seems to be a general feature in A. dispar. Based on the results in A. dispar, males can be suggested as proper sex for morphometric studies.

The present project was aimed at determining the content, quality, and purity of β-carotene extracted from Azolla filiculoides in the Anzali Wetland, comparing it with synthetic β-carotene, and measuring its economic value. One treatment had β-carotene derived from Azolla filiculoides in the Anzali Wetland through the alkaline hydrolysis method in the summer of 2014. Treatments were kept at 4oC for one year. Synthetic β-carotene was used as the control. The quality of the treatments was assessed by applying some chemical tests, including the measurement of the content and quality of β-carotene, colorimetry using the Hunter-LAB method, determination of the purity and vitamin A employing high-performance liquid chromatography (HPLC), estimation of the dwell-time duration at 5oC, and measurement of the solubility of β-carotene in water. The results of the tests regarding the purity, concentration, colorimetry, vitamin compounds, dwell time, and solubility in the experimental β-carotene, compared with those in the control, revealed no significant difference (p>0.05). Moreover, the factors showed no significant difference between the control and experimental treatments during the dwell time (p>0.05). The natural β-carotene had a good quality during the storage period at 5oC for one year. Since there was no significant difference between the β-carotene derived from Azolla filiculoides and the synthetic one in terms of the chemical tests, purity, and dwell time, and since the natural β-carotene derived from Azolla filiculoides takes precedence over the synthetic one in terms of the economic value, it is recommended that natural β-carotene extracted from Azolla filiculoides be substituted for synthetic β-carotene in the food industry.

Harmful algal blooms resulting in red discoloration of coastal waters in the Persian Gulf, Iran were first observed in January 2007. The species responsible for the bloom, which was identified as Cochlodinium polykrikoides, coincided with massive aquatic organisms’ mortalities in the Persian Gulf. The effects of water soluble extract (0.2, 0.6, 0.8 and 1.6 g L-1) from 6 species of marine macroalgae; Ulva lactuca, Enthromorpha intistialis, Colpomenia sinuosa, Sargassum illicifolium, Gracilaria corticat and Hypnea valentiea , fresh thallus (2.5 and 5.0 g L-1) and macroalgal culture medium filtrate from 3species of marine macroalgae; E.intistialis, C.sinuosa, and H.valentiea on growth of Cochlodinium polkrikoides (Dinoflagellate) were investigated for 15 days in co-culture under controlled laboratory conditions. The results clearly showed that the growth of C. polkrikoides was significantly inhibited by the water-soluble extracts of seaweed at relatively all concentrations in contrast to control with any seaweeds extract (p<0.05). The growth inhibition of C. polykrikoides was significantly higher in co-culture with fresh thallus of E.intistialis, C.sinuosa and H.valentiea (p<0.05). In macroalgal culture medium filtrate the highest growth inhibition of C. polykrikoides was obtained in co-culture with E.intistialis and was significantly higher than the other macroalgal culture medium filtrate of seaweeds(p<0.05). Therefore, we could conclude that using the tested seaweeds either as an extract or in co-culture with fresh thallus could be used as an alternative to biological control of C. polykrikoides.

This research was carried out in accordance with identification of effects of different feeding rates on growth indices, feed conversion ratio, survival and body composition in Oriental River prawn (Macrobrachium nipponense). According to this research, 180 oriental river prawns, with mean (±SE) weight about 1.40±0.18 g were counted and distributed in 12 aquariums with 4 treatments and 3 replicates in each treatment cultured with same conditions. Treatments were designed with different rates (1, 2, 3, 4 percent based on biomass). Prawns were fed 5 times a day with commercial diet for 56 days. Finally, growth indices and body composition ratio was calculated for each treatment. The results showed that the Average Final Weight, Weight Gain and Specific Growth Rate had significantly different in the treatment of feeding 3 % per day more than other treatments (p<0.05), However there were no significant difference in Condition Factor between treatments (p>0.05). The lowest Feed Conversion Ratio was observed in treatment 3% feeding per day, and treatments with 3, 4 % feeding per day had the highest Survival Rate with 77.55± 2.6 and 64.37 ± 1.7 % respectively. Results indicate utility of feeding 3 percent during a day (treatment 3) that has a significant difference in the increase of survival rates, reduced feed conversion ratio and increased carcass protein and lipid, with other treatments (p<0.05).

This study focused on zooplankton structure, biodiversity, relationship between a biotic parameters and zooplankton variation, and trophy state of the Persian Gulf Martyrs Lake (Chitgar-Tehran). Based on the lake condition, samples were collected by Juday net at the 5 stations between 2013 and 2014. This study identified 36 zooplankton taxa comprised of Arthropoda (6 genus), Protozoa (6 genus), Rotatoria (20 genus), Nematoda (1 genus), Gastrotricha (2 genus) and Oligochaeta (1 genus). Furthermore, the first record of the freshwater jellyfish Craspedacusta sp. in Iran, with size group of 15-20 mm in diameter in September is reported in the Chitgar lake. The Rotatoria abundance average was measured 61±18 ind.l-1 in the lake. The Nematoda, Gastrotricha and Oligochaeta taxa were measured the lowest abundance (< 1 ind.l-1) in this study. The annual average zooplankton abundance was measured as 72±18 ind.l-1. The PCA displayed, the Rotatoria Trichocerca sp. and Polyarthera sp. were dominated with high components loading, low variance and high abundance. The CCA showed, there was no correlation between Rotatoria abundance and a biotic parameters. Based on the zooplankton structure and bio-indicator, the lake situation is in the meso-oligotrophic category. Thus, it is might be increased eutrophication trend due to no management and no aquatic control in this ecosystem.

In this study that lasted to 8 weeks, was conducted to determine  the effects of dietary supplementation of lysine and  methionine on body biochemical composition and amino acid profile of Sobaity sea bream, Sparidentex hasta. Therefore, 180 juvenile fish with an initial weight of 31.38 ±1.4 g were distributed randomly among eighteen tanks. Fish were fed to satiation three time per day (08:00, 13:00 and 18:00 hours) with formulated diets containing six different levels of dietary methionine and lysine; Diet 1: a control diet without dietary amino acid supplementation; Diet 2: 100% methionine supplementation; Diet 3: 75% methionine and 25% lysine supplementation; Diet 4: 50% methionine and 50% lysine supplementation; Diet 5: 25% methionine and 75% lysine supplementation and Diet 6: 100% lysine supplementation.The results of this study showed Carcass protein content was significantly affected by the amino acid supplements and the highest level of carcass protein observed in fish were fed by diet 3 (P<0.05). Also Increasing levels of  lysine in diet caused increases the amount of fat in the carcass although the content of carcass fat, moister and ash were not significantly different among thetreatments (P>0.05). In addition, essential amino acids (∑EAA) and non-essential amino acids (∑NEAA) and  ratio ∑EAA / ∑NEAA, were affected by lysine and methionine amino acid supplementation as ∑EAA and ratio (∑EAA) / (∑NEAA) significantly increased with increasing levels of amino acid supplementation and the highest amount of this parameters observed in groups were fed by diet with high levels of methionine.The results showed that adding 75%  dietary methionine supplementation and 25% lysine supplemtation to the diet containg 45.95% protein, have positive effects on biochemical composition and amino acid profile in rearing of Sobaity seabream juveniles.

Dam reservoirs have important rules in aquaculture and fish farming in many countries. In this regard, water samples of Azad Dam reservoir (Sanandaj) were collected at five stations from July 2015 to June 2016. Then, the results of 18 physico-chemical parameters were compared with the aquaculture standards and thresholds. Results showed that thermocline and oxycline was started from June and they vanished in December. Maximum level of thermal and oxygen stratified were registered during August and June, respectively. Mean of the most physico-chemical parameters were significantly different between surface and bottom layers (T-Test, p<0.05). Mean of temperature, dissolved oxygen, total alkalinity, total hardness, pH, total suspended solid, total dissolved solid and nutrients were in range of standard threshold of different countries. As a result, based on physico-chemical parameters and with proper management decision, the dam reservoir could be considering suitable for standard pattern of aquaculture activities

The aim of this study was to investigate the effects of different salinity on kidney of Barbus sharpeyi. For this study, 144 healthy Barbus sharpeyi with an average weight of 350±2.36 grams and length 25±1.25 cm in five groups were studied. The first group as control located in municipal dechlorination water and the next four groups respectively were kept in salinity 4ppt, 8ppt, 12ppt and 16ppt in the same condition. On days 1, 3, 7, 14, 21 and 28 sample of kidney with maximum thickness of 0.5 cm prepare and were placed in  bouin's solution.Then the standard method of parafin sections were done and 5- 6 micrometer thick of tissue sections prepared and stained with H&E methods. Results showed the gradual transfer of fish to water with high salinity caused obvious changes as increase the number and diameter of the glomeruli  especially in high salinity but the severity was reduced at the end of the period (p<0.05). Also highest diameter and thickness of the collecting tubules were reported in fresh water at 28 days (p<0.05). Due to the decrease of freshwater resources, from this research can be concluded that the Barbus sharpeyi how much can resist opposite the salinity.These findings suggest that fish Barbus sharpeyi was friendly with salinity and ability to set vital to different salinity.

One of the dominant species of bandar abbas  is (Penaeus indicus) which its propagation is in addition to problems of  propagation attention to environmental, nutritional, health, diseases, parasitics infestation and etc. are important.In this titled : Determination of the frequency of  Infestation of (Penaeus indicus and Litopenaeus  vannamei)  within internal protozoan  Gregarine  , Penaeus indicus samples randomly by traditional fishing with bottom trawl fishing located in the CIRIk (2013) was prepared and samples Litopenaeus vannamei shrimp farms of North Tyab prepared (2013) and sent to the laboratory and the Wet mount preparation method of the digestive tract was investigated.Frequency of examined shrimps according the times of the catch as following: From 100 specimens of the Litopenaeus  vannamei  examined in the year 2013, none  were infected with the protozoan in trophozoite or Gametocystic state. From 100 specimens of the Penaeus indicus shrimp only 31 specimens were infected with Gregarine. 13 individual were female and 18 of them were  male. 31 of these shrimps were infected with parasitic Gregarine as trophozoite or Gametocystic state or both of them, thus their prevalence of infection were 31 percent(trophozoite state: 54.83% and Gametocystic state: 64.51%). Frequency of infestation Trophozoite was 10.29 and Gametocyst was 72.45.It appears that the frequency of infestation with Gregarine increase according to the length of the shrimp. In the length group of 114-133 mm it was 50 percent and in the  length group of 164-185 mm it was 77 percent.

Concentration of chlorophyll-a is an adequate parameter for assessing the trophic state in the summer. So, check the photosynthetic content of pigments as a bioindicator of chlorophylla,b,c were studied in six stations of Gomishan international wetland and a sea station. Furthermore were investigated the qualitative evaluation of trophic state of the wetland and its dynamics. Phytoplankton samples after the filtration were extracted in 90% acetone, and were obtained the absorption of pigments in Spectrophotometer. In addition to chlorophyll, were measured the water temperature, dissolved oxygen, pH, salinity, turbidity and depth. Based on results, the increasing concentrations of chlorophyll-a in September showed the trophic level was hypertrophic and occurred the cyanobacterial bloom. The statistical results showed log Chl-a a positive significant correlation with water temperature, dissolved oxygen and salinity (p<0.01). Also in the Gorganrud estuarine (S2) was measured the diatom bloom as pollution indicator in July. The Gomishan coastal wetland on one hand affected by the human activities in adjacent areas and the other hand under is the influence of global warming on a large scale and in its catchment area, that causing the intensity of evaporation, salinity and become its shallow. Accordingly, improving circulation of wetland water and connection with the sea, improving future guidelines on the estuarine management, reduce and control of urban wastewater and aquaculture wastewater filtration is suggested.

In this study, biochemical heamolymph parameters indices of the white leg shrimp (Litopenaeus vannamei) with three dietry protein levels(25, 35 and 45%) in the three levels of water salinity(0-3, 12-15 and 32-35 ppt) was studied. For experimental 350 shrimps with the average weight of 5.55±0.18 g and length of 8.81±0.15 cm were distributed randomly in 27 ten tones (length: 600 cm, width: 170 cm and height: 100 cm) concrete tanks. Experimental group were fed to satiation 4 times a day the experimental period. The results showed that at dietary protein levels of 35 and 45% and the water salinity of 32-35 ppt the amount of protein, urea, HDL and LDL increased (p≤ 0/05). Cholesterol and triglycerides levels in treatment with dietry protein level of 45% and the salinity of 32-35 ppt had increased. The glucose level of the plasma changed only by the modification of the protein level and had a revers relation with this variable (p≤ 0/05). However, different levels of protein and salinity had no effect on the amount of hemolymph uric acid. So besed biochemical heamolymph parameters on the results of this study, in dietry protein level 35% and salinity of 32-35 ppt and at least stress in the levels.