Purpose: The aims of this study was to examine the effects of moderate and vigorous (60-65% and 80-85% of maximal reserve heart rate respectively) aerobic exercises on the arylesterase activity (ARE ) and total antioxidant capacity (TAC).Method: 44 non-active men volunteered for this research and were divided into three groups randomly, including vigorous aerobic exercise (n= 15; 69.50± 9.29/30.93±6.38 mean weight/age), moderate aerobic exercise (n=17; 76.11± 9.39/34.94±7.44 mean weight/age), and control (n=12; 75.54±7.46/29.75 ±5.31 mean weight/age) group. Duration of training was 8weeks, 3 sessions per weeks and each session lasted 30-45 minutes. Dependent variables were measured in the three phases of the study, including pre-test, mid-test (fourth week) and post-test. TAC and ARE activity measured by Randox kit of UK and enzymatic methods respectively. We applied analysis of variance (ANOV A) test for repeated measures and LSD test for coupled comparisons between times, groups and time-group interaction.Results: Both aerobic exercises did not affect significantly serum ARE activity, TAC and total cholesterol (TC) concentration.However, we observed that HDL-c and HDL-c/TC ratio significantly increase in vigorous aerobic exercise group after 8 weeks (P<0.05). Besides, we observed that maximal oxygen uptake (Vo2max) increased significantly in both vigorous erobic exercise (42.78± 4.90 vs. 50.80±6.77) and moderate aerobic exercise group (37.90± 5.32 vs. 43.88± 6.17) after 8 weeks (P<0.05), while it remained without significant changes (41.2l± 5.88 vs. 42.98± 5.87) in control group. Further more there were significant positive relationships among Vo2max with TAC (r=0.36, P<0.01) and HDL-c (r=0.30, P<0.04).Conclusion: Overall, our results suggest that the moderate and vigorous aerobic exercises (with intensities60 to 85 percent of maximal reserve heart rate) could not produce oxidative stress, and in turn ARE activityor TAC. However, they, especially vigorous exercises, modified lipid profile beneficially. Correlation results suggest that improved physical fitness with be associated with higher antioxidant defense system and lower atherogenic risk factors.