Introduction: Molar-incisor hypomineralization (MIH) is a rapidly destructive type of enamel defects of systemic origin. The aim of the present study was to investigate the prevalence of MIH and the potential predictive factors among the children within the age group of 6-13 years in Rasht, Iran.Materials & Methods: The prevalence of MIH was determined through clinical examination of 1043 children aged 6-13 years. At In the next stage, 235 subjects with MIH and their matched controls were evaluated for determining the probable etiologic risk factors. The data were collected using questionnaire and interviews entailing some questions about the late pregnancy problems, birth time problems, and the diseases at the first three years of life. The data analysis was performed using the Chi-square, Fisher’s exact, and Mann-Whitney U tests through the SPSS version 17. The p-vale less than 0.05 was considered statistically significant.Results: According to the results of the study, the prevalence of MIH was 19.9%, and the sole molar involvement was 17.4%. On average, every child had 2.18 hypomineralized teeth involving 1.9 molars and 0.28 incisor. There was no significant difference between the two genders in terms of the prevalence and intensity of the MIH (P=0.54, X2=0.36). Furthermore, no significant difference was observed between the private and public school students regarding the prevalence of this defect (P=0.51, X2=0.47). As the results indicated, the enamel defects were more common in the lower molars, compared to those in the upper ones (P<0.001, X2=19.6). The most common type of defect was opacities. Moreover, no association was found between the MIH and the investigated variables.Conclusion: As the findings of the present study revealed, the MIH had a high prevalence. However, most of the defects were mild, and the mean of the MIH-affected teeth was two teeth per child. The opacities were the main type of defect. None of the investigated variables was associated with the MIH. Further studies are recommended to investigate the etiologic factors including the genetic background to determine the predisposing factors.