Mercury concentration in the muscle, intestine, kidney and gonad of Chalcalburnus chalcalburnus from Anzali wetland was determined in order to compare with international standard levels and also to determine the influence of weight, age and sex on the accumulation of this element in fish tissues. The tissue sample was separated from 23 specimens. Determination of mercury was done by AMA, 254, LECO (Advanced Mercury Analyzer), Standard Method No. D-6722. The mean concentrations of mercury were 70.73, 119.71, 158.69 and 189.13 ppb dry weight in muscle, intestine, gonad and kidney, respectively.No significant difference between age and mercury values was found (p=0.26 for muscle, p=0.15 for intestine, p=0.39 for kidney and p=0.30 for gonad). The same results were also found between sex, length and mercury concentration, but no significant difference was found between mercury concentration in muscle, kidney and gonad and the variable of weight (p=023 for muscle, p=0.13 for kidney and p=0.72 for gonad). There was, however, a significant difference between mercury values in intestine and the variable of weight at confidence level of 95% (p=0.03). Comparing mercury concentration in muscle (70.73 ppb) with standard threshold levels, it was lower than that of WHO (500 ppb), FAO (500 ppb) and FDA (1000 ppb).