Aluminum is one of the most abundant elements in the earth crust and enter to the body through drinking water ,industrial use, nutrients and antacids. Aluminum poisoning causes wide range of disorders. Because the renal failure patient with high serum aluminum have anemia. This experiment was performed to study the effect of aluminum on blood factors in rat. The experiment performed in four groups of male rats (n=13). In addition of control group, three groups of animals, consumed a diet containing aluminum (0.625, 1.25 and 2.5 mg/gram diet) for 60 days. At the end, animals were sacrificed and blood samples were collected. RBC counted by hemocytometer, hemoglobin and hematocrit were measured by usually laboratory methods and then MCHC was justified. Results are expressed as mean±SE and P<0.05 were significant. The results show that RBC, hemoglobin, hematocrit and MCHC decreased in those groups which consumed the diet containing 2.5 milligram aluminum compared to control group.These results indicated that high aluminum intake in rat causes anemia, decreases RBC, hemoglobin, hematocrit and MCHC in rat.