Olive leaf spot or peacock spot caused by the fungal pathogen Spilocaea oleaginea, can cause reduced growth and yield, bringing in economical loss to olive trees and orchards. A survey of olive leaf spot and an evaluation of resistance of 25 cultivars to this disease were carried out during 2003 to 2005 in Mazandaran province. According to the obtained results the degree of infection and severity of olive leaf spot was low in areas that were to a larger extent exposed to wind and dry conditions (hills and rivers banks), while it was very high in areas where leaves of olive trees were exposed to calm weather with high rainfall and humid conditions (north side of hills). Any investigations of different ages of olive trees showed that there was a positive correlation between disease severity and increased age. Most 2-3 year young trees were resistant to very resistant and a majority of 10-12 years trees were susceptibleto very susceptible. An evaluation of olive cultivars' resistance showd that Manzanilla, Roughani (Roudbar), Zard, Kalamon, Sevillano, Mission, Amygdaloidal, Karolea, Konservolia, Koroneiki, Frangivento, Roughani (mohaly Gargan), Ascolana, Coratina, and Velanonia were susceptible, while Arbequina, Nabali Balady and Moraiolo semi susceptible, and Mary, Frantoio, Rosso, Simarimella, Picual, Leccino, and Dolce Agogia being resistant. With a consideration of the disease severity in different parts of the region, it is proposed that seven resistant cultivars (Mary, Frantoio, Rosso, Simarimella, Picual, Leccino and Dolce Agogia) to peacock spot disease be used for cultivation.