This qualitative research was carried out to identify barriers to development of agro-enterprises. The required information was collected, based on the framework of research protocol and twelve leading questions, by a semi- structured interview and a sample of 43 entrepreneurs and managers of small agro-enterprises. The content of interviews were analyzed by content analysis technique and the results of this process was the extraction of 107 items representing the barriers to development of agro-enterprises. A complete list of items was categorized, in relation to their conceptual affinity, to five thematic classes as follows: natural factors, production factors, complexity of nature and process of agro-businesses, development of agricultural markets, and environment and context of agro-enterprises. The results indicated that the following barriers have the highest frequency: incidence of natural calamities, low quality of existing inputs in the markets, time lag between making the initial investment and profit realization, fluctuations in agricultural markets in relation to imports, exports, pricing policies, and market regulation, and difficult requirements to access bank credits.