Antimicrobial Activities of the Secondary Metabolite Extracted from a Nocardia Strain

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Seratnahaei Marjan; ESHRAGHI SEYYED SAEED; PAKZAD PARVIZ; ZAHRAEI RAMAZANI ALIREZA; YASERI MEHDI

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Journal Paper

Paper Information

Journal: Journal of Advanced Biomedical Sciences Year:2022 | Volume:12 | Issue:2 Page(s): 203-214

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Title

Antimicrobial Activities of the Secondary Metabolite Extracted from a Nocardia Strain

Author(s)

Keywords

Nocardia soli

antimicrobial activity

HPLC

Bioactive metabolite

Abstract

Background & Objective: The emergence of antibiotic-resistant bacteria and limited treatment options for these infections is a challenging problem in the world. There is increasing interest in isolating bioactive molecules from actinobacteria and elucidating their antimicrobial mechanisms at the moment. The current study aimed to investigate the antimicrobial activities of a Nocardia strain (N4) that was previously isolated from the soil samples in Tehran, Iran. Materials & Methods: The cultural, morphological, and physiological characteristics of the strain were determined and the identity of the strain was confirmed using 16SrRNA gene sequencing. The strain was screened for antimicrobial metabolite production. The in vitro anti-microbial activity of the extracted metabolite was assessed by agar well diffusion and minimum inhibitory concentrations methods. Finally, the structure of the extracted metabolite was confirmed by HPLC and mass spectrometry methods. Results: The isolate shared 99% sequence similarity with Nocardia soli. The strain showed broad-spectrum antimicrobial activities against some pathogenic bacteria (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 700603, Salmonella Typhimurium ATCC 14028 and Shigella sonnei RI366) and fungi (Aspergillus niger ATCC 1015 and Aspergillus fumigatus ATCC 1022) in the agar well diffusion method. The ethyl acetate extract of the strain exhibited the highest antimicrobial activity against E. coli (MIC= 4. 67±, 3. 055 μ, g/mL) and K. pneumoniae (MIC=3. 33±, 1. 155 μ, g/mL). The metabolite showed high similarity with C2 1H28 N7 O14. Conclusions: The current study confirmed the presence of a Bioactive metabolite in strain N4, suggesting that this may be a promising candidate for discovering bioactive components with broad antibacterial activities against a variety of pathogens.

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References

Characterization of Streptomyces Isolates Associated with Estuarine Fish Chanos chanos and Profiling of Their Antibacterial Metabolites-Crude-Extract

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APA: Copy

Seratnahaei, Marjan, ESHRAGHI, SEYYED SAEED, PAKZAD, PARVIZ, ZAHRAEI RAMAZANI, ALIREZA, & YASERI, MEHDI. (2022). Antimicrobial Activities of the Secondary Metabolite Extracted from a Nocardia Strain. JOURNAL OF ADVANCED BIOMEDICAL SCIENCES, 12(2), 203-214. SID. https://sid.ir/paper/1000406/en

Vancouver: Copy

Seratnahaei Marjan, ESHRAGHI SEYYED SAEED, PAKZAD PARVIZ, ZAHRAEI RAMAZANI ALIREZA, YASERI MEHDI. Antimicrobial Activities of the Secondary Metabolite Extracted from a Nocardia Strain. JOURNAL OF ADVANCED BIOMEDICAL SCIENCES[Internet]. 2022;12(2):203-214. Available from: https://sid.ir/paper/1000406/en

IEEE: Copy

Marjan Seratnahaei, SEYYED SAEED ESHRAGHI, PARVIZ PAKZAD, ALIREZA ZAHRAEI RAMAZANI, and MEHDI YASERI, “Antimicrobial Activities of the Secondary Metabolite Extracted from a Nocardia Strain,” JOURNAL OF ADVANCED BIOMEDICAL SCIENCES, vol. 12, no. 2, pp. 203–214, 2022, [Online]. Available: https://sid.ir/paper/1000406/en

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