Lack of effect of plasma of myocardial preconditioned, ischemic and ischemic-reperfused rats of myocardium on differentiation of mesenchymal stem cells into cardiomyocytes

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ESMAILIDEHAJ MANSOUR; Esmaeili Hassan; CHAVOSHI ELHAM; REZVANI MOHAMMAD EBRAHIM; Azizian Hossein

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Journal: Journal of Shahid Sadoughi University of Medical Sciences Year:2022 | Volume:30 | Issue:4 Page(s): 4778-4792

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Title

Lack of effect of plasma of myocardial preconditioned, ischemic and ischemic-reperfused rats of myocardium on differentiation of mesenchymal stem cells into cardiomyocytes

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Keywords

Mesenchymal stem cells

ischemia preconditioning

plasma

Desmin

-MHC and troponin I

Abstract

Introduction: Numerous studies have shown that enriched plasma protects myocardial cells against ischemia. The aim of this study was to evaluate the effect of rat enriched plasma by preconditioning, ischemia, and reperfusion on the differentiation of bone marrow Mesenchymal stem cells (BMMSC) into cardiomyocytes. Methods: In this experimental and laboratory study, BMMSCs were extracted from the femur bone of male Wistar rats weighting 250-300 g. BMMSCs of the third passage were divided into six groups according to the type of plasma received: 1) control, 2) azacytidine, 3) azacytidine and non-ischemic plasma recipient, 4) azacytidine and preconditioned plasma recipient, 5) azacytidine and ischemic plasma recipient 6) azacytidine and plasma reperfusion recipient groups. In all groups except group 1, to initiate cardiomyocyte differentiation, BMMSCs were exposed to azacytidine (10 μ, M) for 24 hours. plasma (25 μ, g / ml) was added to the culture medium each time the culture medium was changed. After 21 days, the expression level of myocardial β, MHC, Desmin, and troponin I genes was assessed using qRT-PCR. Results: The expression of myocardial β, MHC, Desmin, and troponin I genes was significantly increased in all groups received azacytidine (groups 2 to 6) (P<0. 01). There was no significant difference in the expression of myocardial β, MHC, Desmin, and troponin I genes, the doubling time, and migration between the plasma receiving groups (groups 3 to 6) compared to the group 2 (P>0. 05). Conclusion: The findings of present study showed that the plasma collected during ischemia, ischemiareperfusion, and ischemia preconditioning had no effect on the differentiation of BMMSCs into cardiomyocytes in the presence of azacytidine.

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APA: Copy

ESMAILIDEHAJ, MANSOUR, Esmaeili, Hassan, CHAVOSHI, ELHAM, REZVANI, MOHAMMAD EBRAHIM, & Azizian, Hossein. (2022). Lack of effect of plasma of myocardial preconditioned, ischemic and ischemic-reperfused rats of myocardium on differentiation of mesenchymal stem cells into cardiomyocytes. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, 30(4 ), 4778-4792. SID. https://sid.ir/paper/1017202/en

Vancouver: Copy

ESMAILIDEHAJ MANSOUR, Esmaeili Hassan, CHAVOSHI ELHAM, REZVANI MOHAMMAD EBRAHIM, Azizian Hossein. Lack of effect of plasma of myocardial preconditioned, ischemic and ischemic-reperfused rats of myocardium on differentiation of mesenchymal stem cells into cardiomyocytes. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2022;30(4 ):4778-4792. Available from: https://sid.ir/paper/1017202/en

IEEE: Copy

MANSOUR ESMAILIDEHAJ, Hassan Esmaeili, ELHAM CHAVOSHI, MOHAMMAD EBRAHIM REZVANI, and Hossein Azizian, “Lack of effect of plasma of myocardial preconditioned, ischemic and ischemic-reperfused rats of myocardium on differentiation of mesenchymal stem cells into cardiomyocytes,” JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, vol. 30, no. 4 , pp. 4778–4792, 2022, [Online]. Available: https://sid.ir/paper/1017202/en

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