MOLECULAR TAGGING OF COLD-INDUCIBLE GENES IN AGROPYRON DESERTORUM AND A. ELONGATUM

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TABAEI AGHDAEI S.R.

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Paper Information

Journal: Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research Year:2001 | Volume:- | Issue:6 Page(s): 43-55

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Title

MOLECULAR TAGGING OF COLD-INDUCIBLE GENES IN AGROPYRON DESERTORUM AND A. ELONGATUM

Author(s)

TABAEI AGHDAEI S.R. | Issue Writer Certificate

Keywords

COLD HARDINESSQ4

GENE EXPRESSIONQ4

LOW-NON-FREEZING TEMPERATUREQ4

STREES-INDUCIBLE GENESQ4

MRNA ACCUMULATIONQ4

SURVIVALQ4

NORTHERN HYBRIDIZATIONQ4

AGROPYRON DESERTURUM AND A. ELONGATUMQ4

Abstract

Differential expression of low - temperature - induced genes was investigated in Agropyron desertorum and A.elongatum. Total RNA was extracted from the plants grown under 20/15°C (day/night) and at 6/2°C (day/night) temperatures. The RNA was then fractionated through electrophoresis, and accumulation of mRNAs corresponding to stress - inducible nsLTP and dehybrin gene families was analyzed, using NORTHERN HYBRIDIZATION.The mRNA level of the test genes increased in plants under 6/2°C, indicating GENE EXPRESSION enhancement in responses to low temperature stress. Also, COLD HARDINESS was examined through a frost test, by assessment of SURVIVAL in both control (20/15°C) and low - temperature (6/2°C) -treated plants after freezing. Freezing tolerance was improved in plants grown at LOW-NON-FREEZING TEMPERATUREs. Also, the levels of freezing SURVIVAL and GENE EXPRESSION were higher in A. desertorum than in A. elongatum. A positive correlation was also observed between the degree of freezing tolerance and the exprossion of the cold-inducible genes, and this could be a useful result in clarification of the role of the genes in plant freezing tolerance.

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APA: Copy

TABAEI AGHDAEI, S.R.. (2001). MOLECULAR TAGGING OF COLD-INDUCIBLE GENES IN AGROPYRON DESERTORUM AND A. ELONGATUM. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, -(6), 43-55. SID. https://sid.ir/paper/104243/en

Vancouver: Copy

TABAEI AGHDAEI S.R.. MOLECULAR TAGGING OF COLD-INDUCIBLE GENES IN AGROPYRON DESERTORUM AND A. ELONGATUM. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH[Internet]. 2001;-(6):43-55. Available from: https://sid.ir/paper/104243/en

IEEE: Copy

S.R. TABAEI AGHDAEI, “MOLECULAR TAGGING OF COLD-INDUCIBLE GENES IN AGROPYRON DESERTORUM AND A. ELONGATUM,” IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, vol. -, no. 6, pp. 43–55, 2001, [Online]. Available: https://sid.ir/paper/104243/en

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