Optimization of regeneration in Mountain Ziziphora (Ziziphora clinopodioides Lam)

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MOMENI A.; ZAKER TAVALLAIE F.; Shokoohifar F.; KHEIRKHAH M.

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Paper Information

Journal: Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research Year:2019 | Volume:27 | Issue:1 (53) Page(s): 143-151

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Information Journal Paper

Title

Optimization of regeneration in Mountain Ziziphora (Ziziphora clinopodioides Lam)

Author(s)

MOMENI A. | ZAKER TAVALLAIE F. | Shokoohifar F. | KHEIRKHAH M. | Issue Writer Certificate

Keywords

MicropropagationQ2

Multiple shoot inductionQ2

RootingQ1

Ziziphora clinopodioides LamQ1

Abstract

Medicinal and aromatic medicinal plant, Ziziphora clinopodioides L., belonging to the genus Ziziphora and Lamiaceae family. The aim of this study was to optimize asexual regeneration in two genotypes of this species as code 5 and 6. For this purpose, the first experiment was conducted for contamination control using sodium hypochlorite 1% and alcohol 96%. In the next step, the control of produced phenolic compounds was investigated by combining three polyvinylpyrrolidone, active charcoal and ascorbic acid. Multiple shoots induction were performed using lateral bud explant in a MS medium containing the BAP at five levels in combination with NAA (0. 01 mg/L). Rooting of branches was performed in MS medium containing NAA at two levels (0. 1 and 0. 3 mg/L). Result showed 1% sodium hypochlorite treatment coupled with 96% alcohol eliminated contamination up to 74%. The treatment of combination of polyvinylpyrrolidine, ascorbic acid and activated charcoal with average value of 8. 25% had the lowest browning in both Genotype of 6 and 5. The highest branching values of (88% and 100%) were obtained in the treatment of 2 and 2. 5 mg/L BAP combined with 0. 01 mg/L NAA in genotype 5 and 6, respectively. The highest value of Rooting (33. 3%) was observed in genotype 6 using 0. 3 mg/l NAA. The results of this research could be used in the Micropropagation and proliferation of this plant.

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APA: Copy

MOMENI, A., ZAKER TAVALLAIE, F., Shokoohifar, F., & KHEIRKHAH, M.. (2019). Optimization of regeneration in Mountain Ziziphora (Ziziphora clinopodioides Lam). IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, 27(1 (53) ), 143-151. SID. https://sid.ir/paper/104269/en

Vancouver: Copy

MOMENI A., ZAKER TAVALLAIE F., Shokoohifar F., KHEIRKHAH M.. Optimization of regeneration in Mountain Ziziphora (Ziziphora clinopodioides Lam). IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH[Internet]. 2019;27(1 (53) ):143-151. Available from: https://sid.ir/paper/104269/en

IEEE: Copy

A. MOMENI, F. ZAKER TAVALLAIE, F. Shokoohifar, and M. KHEIRKHAH, “Optimization of regeneration in Mountain Ziziphora (Ziziphora clinopodioides Lam),” IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, vol. 27, no. 1 (53) , pp. 143–151, 2019, [Online]. Available: https://sid.ir/paper/104269/en

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