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Information Journal Paper

Title

AN HPLC METHOD FOR DETERMINATION OF MEBUDIPINE IN BIOLOGICAL FLUIDS IN RATS AND ITS APPLICATION IN PHARMACOKINETIC STUDY

Pages

  41-47

Abstract

 Background & Aim: Mebudipine is a calcium antagonist drug which is used to treat blood pressure. A new high performance liquid chromatography(HPLC) system for the determination of a new 1, 4-dihydropyridine, mebudipine, in rat plasma and other biological fluids was developed in this study for the first time.Materials & Methods: To 1 ml of rat plasma and/or other biological fluids, 0.5 ml of internal standard(dibudipine) and 0.5 ml of 1 M NaOH was added. Mebudipine and internal standard were extracted to 5 ml ethyl acetate, evaporated under slow stream of nitrogen. The residue was reconstituted in 200μl mobile phase and 20μl of aliquots was injected into a HPLC system equipped with 4.6×250mm i.d.C18 analytical column. Mobile phase consisted of methanol(70%), water(25%) and acetonitril(5%) and its flow rate was 1 ml/min.Results: There were no interfering peaks from endogenous components in blank plasma chromatograms. Standard curves were linear(r(2)>0.99) over 10 to 500ng/ml. The extraction efficiency was above 90% and the minimum quantifiable concentration was 10ng/ml(CV<10%) which reveals that it is a suitable, convenient and simple HPLC assay for pharmacokinetic study of mebudipine in rat.Conclusion: By using this method, it was found out that while the oral bioavailability of mebudipine was low(<2%), it had a marked first-pass effect. The distribution of mebudipine into some tissues such as brain, heart, liver and kidney following intravenous administration(0.5 mg/kg) was studied and a rapid distribution of mebudipine into these tissues was found. It was concluded that brain, heart, liver and kidney are in the same compartment as plasma(central).

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    APA: Copy

    BOHLOULI, SH., KEYHANFAR, F., ZIAEE, S., & MAHMOUDIAN, M.. (2005). AN HPLC METHOD FOR DETERMINATION OF MEBUDIPINE IN BIOLOGICAL FLUIDS IN RATS AND ITS APPLICATION IN PHARMACOKINETIC STUDY. RAZI JOURNAL OF MEDICAL SCIENCES (JOURNAL OF IRAN UNIVERSITY OF MEDICAL SCIENCES), 12(48 (SPECIAL ISSUE)), 41-47. SID. https://sid.ir/paper/10434/en

    Vancouver: Copy

    BOHLOULI SH., KEYHANFAR F., ZIAEE S., MAHMOUDIAN M.. AN HPLC METHOD FOR DETERMINATION OF MEBUDIPINE IN BIOLOGICAL FLUIDS IN RATS AND ITS APPLICATION IN PHARMACOKINETIC STUDY. RAZI JOURNAL OF MEDICAL SCIENCES (JOURNAL OF IRAN UNIVERSITY OF MEDICAL SCIENCES)[Internet]. 2005;12(48 (SPECIAL ISSUE)):41-47. Available from: https://sid.ir/paper/10434/en

    IEEE: Copy

    SH. BOHLOULI, F. KEYHANFAR, S. ZIAEE, and M. MAHMOUDIAN, “AN HPLC METHOD FOR DETERMINATION OF MEBUDIPINE IN BIOLOGICAL FLUIDS IN RATS AND ITS APPLICATION IN PHARMACOKINETIC STUDY,” RAZI JOURNAL OF MEDICAL SCIENCES (JOURNAL OF IRAN UNIVERSITY OF MEDICAL SCIENCES), vol. 12, no. 48 (SPECIAL ISSUE), pp. 41–47, 2005, [Online]. Available: https://sid.ir/paper/10434/en

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