APPLICATION OF SNAIL DIGESTIVE SYSTEM EXUDATES IN CYTOGENETIC STUDIES

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ABDI GHAZI JAHANI A.; MIRZAEI NODUSHAN H.; RAZBAN HAGHIGHI A.; IMANI Y.; JAVADI MAMAGHANI H.

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Journal: Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research Year:2003 | Volume:11 | Issue:4 Page(s): 421-428

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Title

APPLICATION OF SNAIL DIGESTIVE SYSTEM EXUDATES IN CYTOGENETIC STUDIES

Author(s)

Keywords

SNAIL DIGESTIVE SYSTEM EXUDATESQ4

SNAIL SP. (HELIX SP.)Q4

BIOLOGIC DIGESTIONQ4

CYTOGENETICQ3

AGROPYRON TAURIQ3

Abstract

In CYTOGENETIC studies, preparing well scattered chromosomes within mitotic cells in microscopic specimens for a high resolution is always a bottle neck for cytologists. To overcome this problem, SNAIL DIGESTIVE SYSTEM EXUDATES was used instead of commercial enzymes which are routinely used in order to digest the mitotic cell organelles for preparing transparent mitoticcells. This was done to investigate the possibility of substitution of an endemic and easily accessible tool for the commercial enzyme. To extract the exudates, snails were collected from Arasbaran forests and the digestivesystem exudates were extracted. The exudates were centrifuged for 30 minutes at 4000 RPM and the supernatants were kept at zero degree centigrade. In microscopic studies on the species of AGROPYRON TAURI the exudates was applied on root meristem after pretreatment, hydrolyze and staining processes which caused a uniform scattering of the chromosomes on the mitotic cell plate.

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APA: Copy

ABDI GHAZI JAHANI, A., MIRZAEI NODUSHAN, H., RAZBAN HAGHIGHI, A., IMANI, Y., & JAVADI MAMAGHANI, H.. (2003). APPLICATION OF SNAIL DIGESTIVE SYSTEM EXUDATES IN CYTOGENETIC STUDIES. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, 11(4), 421-428. SID. https://sid.ir/paper/104358/en

Vancouver: Copy

ABDI GHAZI JAHANI A., MIRZAEI NODUSHAN H., RAZBAN HAGHIGHI A., IMANI Y., JAVADI MAMAGHANI H.. APPLICATION OF SNAIL DIGESTIVE SYSTEM EXUDATES IN CYTOGENETIC STUDIES. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH[Internet]. 2003;11(4):421-428. Available from: https://sid.ir/paper/104358/en

IEEE: Copy

A. ABDI GHAZI JAHANI, H. MIRZAEI NODUSHAN, A. RAZBAN HAGHIGHI, Y. IMANI, and H. JAVADI MAMAGHANI, “APPLICATION OF SNAIL DIGESTIVE SYSTEM EXUDATES IN CYTOGENETIC STUDIES,” IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, vol. 11, no. 4, pp. 421–428, 2003, [Online]. Available: https://sid.ir/paper/104358/en

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