Evaluation of SO6 protein in Saponaria officinalis L. callus

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HONARI H.; Etemad Aubi s.m.; ESMAILI F.; ABDOLLAHI M.

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Journal Paper

Paper Information

Journal: Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research Year:2018 | Volume:26 | Issue:1 (51) Page(s): 23-31

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Information Journal Paper

Title

Evaluation of SO6 protein in Saponaria officinalis L. callus

Author(s)

HONARI H. | Etemad Aubi s.m. | ESMAILI F. | ABDOLLAHI M. | Issue Writer Certificate

Keywords

CallusQ1

growth regulatorsQ1

Saponaria officinalis L.Q1

SO6 proteinQ2

Abstract

Soapwort (Saponaria officinalis L. ) is a native plant to central and southern Europe. It is an important and rare plant species in Iran. The species has numerous applications in medicinal, agricultural and health fields. Saponins is one of the bioactive compounds in the species that is used in chemotherapy. Tissue culture is one of the ways of proliferation of the species that is used for production of active ingredients. Saponin has ten isoforms that are different to inhibition of translating ribosomes and cytotoxicity. SO6 is one of the isoforms of saponins. Presence of SO6 protein in soapwort Callus was studied through plant tissue culture and different concentrations of growth regulators and environmental factors. After achieving success in tissue culture, protein was extracted from its Callus and presence of SO6 protein was confirmed by western blot analysis and measured by ELISA. Results showed that seed germination was possible through using appropriate treatment and soapwort root Callus had the highest amount of SO6 protein.

Cites

Sub Cloning and Expression of SO9 gene, Saponaria Officinalis L Plant in E. Coli and Investigation of Antibody Titer in Mouse

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APA: Copy

HONARI, H., Etemad Aubi, s.m., ESMAILI, F., & ABDOLLAHI, M.. (2018). Evaluation of SO6 protein in Saponaria officinalis L. callus. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, 26(1 (51) ), 23-31. SID. https://sid.ir/paper/104395/en

Vancouver: Copy

HONARI H., Etemad Aubi s.m., ESMAILI F., ABDOLLAHI M.. Evaluation of SO6 protein in Saponaria officinalis L. callus. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH[Internet]. 2018;26(1 (51) ):23-31. Available from: https://sid.ir/paper/104395/en

IEEE: Copy

H. HONARI, s.m. Etemad Aubi, F. ESMAILI, and M. ABDOLLAHI, “Evaluation of SO6 protein in Saponaria officinalis L. callus,” IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, vol. 26, no. 1 (51) , pp. 23–31, 2018, [Online]. Available: https://sid.ir/paper/104395/en

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