RAPID METHOD WITH HIGH EFFICIENCY TO TRANSFORM ALFALFA FOR EXPRESSION OF BANYULS (BAN) GENE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HESAMZADEH HEJAZI S.M.; ABD MISHANI S.; ARCIONI S.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research Year:2005 | Volume:13 | Issue:3 (21) Page(s): 295-322

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

1,021

Download:

Cites:

Information Journal Paper

Title

RAPID METHOD WITH HIGH EFFICIENCY TO TRANSFORM ALFALFA FOR EXPRESSION OF BANYULS (BAN) GENE

Author(s)

HESAMZADEH HEJAZI S.M. | ABD MISHANI S. | ARCIONI S. | Issue Writer Certificate

Keywords

ALFALFAQ4

AGROBACTERIUM TUMEFACIENSQ2

ARABIDOPSIS THALIANAQ2

BAN GENEQ2

PROANTHOCYANINIDINS (CT)Q4

REGENERATION AND TRANSFORMATIONQ4

Abstract

Condensed tannins (CTS) are flavonoid oligomers, many of which have beneficial effects on animal (safe bloat) and human health. The functions of CT are due to their ability to form reversible complex with proteins, the formation of these protein-tannin complexes making protein unavailable for microbial enzyme and activate other mechanisms like deprivation of metal ions and inhibition of oxidative phosphorylation which also results in the prevention of foam formation and bloating. Anthocyanins are flavonoids that contribute to the red, violet or blue pigmentation of both seeds and flowers. The BAN GENE encodes anthocyanidin reductase (ANR), an enzyme proposed to convert anthocyanidins to their corresponding 2, 3-cis -flavan - 3-ols ((+)-catechin). Transformation of leaf explants from Medicago sativa var. Rgsy27 and M.sativa var. PI genotypes was carried out with the plasmid pBI121.BAN (binary vector containing the BAN GENE) by two strains of Agrobacterium tumefadens (LBA 4404 & ERA 105). Leaf explants of Rgsy27 produced more than 90% proembryogenic calli, but in PI produced about 30%. More than 65% of regenerated embryos were changed to whole plants in both genotypes. The efficiency of transformation in Rgsy27 and PI plants was depended on Agrobacterium strains, so that the strain LBA 4404 was efficient more than ERA 105 for transformation of both genotypes. Ectopic expression of the BAN in ALFALFA transgenic foliage resulted in accumulation of CTs. Thus, it has been assumed that the BAN GENE also acts as starter units in tannins condensation in ALFALFA. Loss of the BAN GENE function increases the amount of red pigment, suggesting that the BANYULS protein might be a negative regulator of pigment production.BANYULS activity is limiting factor for CT production, making this enzyme an attractive target for induction of over expression in forage plants, such as ALFALFA (Medicago sativa) with the goal of inducing CT production in their foliage. The condensed tannin percentage in dry matter of transgenic Rgsy27 and PI plants was 0.474 and 0.600, respectively and 0.259 and 0.408 in control plants.

Cites

No record.

References

روش تراریزش سریع با کارایی بالا در یونجه های دیپلوئید و تتراپلوئید برای تظاهر ژن های Sn و بتا– گلو کورونیداز (gus)

Cite

APA: Copy

HESAMZADEH HEJAZI, S.M., ABD MISHANI, S., & ARCIONI, S.. (2005). RAPID METHOD WITH HIGH EFFICIENCY TO TRANSFORM ALFALFA FOR EXPRESSION OF BANYULS (BAN) GENE. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, 13(3 (21)), 295-322. SID. https://sid.ir/paper/104576/en

Vancouver: Copy

HESAMZADEH HEJAZI S.M., ABD MISHANI S., ARCIONI S.. RAPID METHOD WITH HIGH EFFICIENCY TO TRANSFORM ALFALFA FOR EXPRESSION OF BANYULS (BAN) GENE. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH[Internet]. 2005;13(3 (21)):295-322. Available from: https://sid.ir/paper/104576/en

IEEE: Copy

S.M. HESAMZADEH HEJAZI, S. ABD MISHANI, and S. ARCIONI, “RAPID METHOD WITH HIGH EFFICIENCY TO TRANSFORM ALFALFA FOR EXPRESSION OF BANYULS (BAN) GENE,” IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, vol. 13, no. 3 (21), pp. 295–322, 2005, [Online]. Available: https://sid.ir/paper/104576/en

Related Journal Papers