IN VITRO PROPAGATION OF THUJA ORIENTALIS BY SHOOT TIP CULTURE

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EMAM MITRA

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Journal Paper

Paper Information

Journal: Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research Year:2003 | Volume:11 | Issue:1 Page(s): 1-15

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Information Journal Paper

Title

IN VITRO PROPAGATION OF THUJA ORIENTALIS BY SHOOT TIP CULTURE

Author(s)

EMAM MITRA | Issue Writer Certificate

Keywords

SHOOT TIP CULTUREQ4

THUJA ORIENTALISQ4

IN VITRO PROPAGATIONQ4

TISSUE CULTUREQ2

SHOOT REGENERATION AND RHYZOGENESEQ3

Abstract

Thuja orientalis is an important forest tree species for timber and wood production. Its wood is light in weight and highly resistant to decay. Regarding difficulties in conventional propagation of Conifers, specifically hard rooting f cutting in adult trees this study was carried out For investigation of asexual regeneration in THUJA ORIENTALIS apical buds from adult trees and seedlings were collected in 3 seasons. After presterilization, the best method for sterilizing of buds was identified. The method was: Cleaning and brushing the buds between and ethanol 70% solution as the pretreatment, dipping them in ethanole 70% for 1minutes and cleaning buds with 0.1% HgCl2 solution in different periods (4 for spring, 6 for summer, and 9 minutes for fall). The suitable medium for establishment was DKW medium with 0.5 mg/lit 2iP. Established explants were subculture in 3 media (MS, DKW and SH) containing 2iP and BA alone or in combination (from 0 to 0.5 mg/lit) + IBA (0.01 mg/lit). After two sub culturing of buds in the media, the means of shoot proliferation and shoot length growth was used as the data for analysis of variance. The best medium for shoot regeneration was the DKW with 0.5 mg/lit 2iP, 0.01 mg/lit IBA (83%). Rhyzogenese of shoots was done in DKW medium supplemented with 2 mg/lit IBA (33%).

Cites

SURVEY OF GROWTH, SURVIVAL AND GERMINATION CHARACTERISTICS OF SEEDS AND SEEDLINGS OF THUJA ORIENTALIS IN DIFFERENT TREATMENTS OF SOWING DATE, IRRIGATION AND WEED CONTROL

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APA: Copy

EMAM, MITRA. (2003). IN VITRO PROPAGATION OF THUJA ORIENTALIS BY SHOOT TIP CULTURE. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, 11(1), 1-15. SID. https://sid.ir/paper/104619/en

Vancouver: Copy

EMAM MITRA. IN VITRO PROPAGATION OF THUJA ORIENTALIS BY SHOOT TIP CULTURE. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH[Internet]. 2003;11(1):1-15. Available from: https://sid.ir/paper/104619/en

IEEE: Copy

MITRA EMAM, “IN VITRO PROPAGATION OF THUJA ORIENTALIS BY SHOOT TIP CULTURE,” IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, vol. 11, no. 1, pp. 1–15, 2003, [Online]. Available: https://sid.ir/paper/104619/en

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