CRYOPRESERVATION OF ACER MONSPESSULANUM SEEDS

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HATAMI FIROUZEH; JEBELI MARYAM; NADERI SHAHAB M.A.; TABARI MASOUD

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Journal Paper

Paper Information

Journal: Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research Year:2010 | Volume:18 | Issue:1 (35) Page(s): 12-23

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Information Journal Paper

Title

CRYOPRESERVATION OF ACER MONSPESSULANUM SEEDS

Author(s)

HATAMI FIROUZEH | JEBELI MARYAM | NADERI SHAHAB M.A. | TABARI MASOUD | Issue Writer Certificate

Keywords

CRYOPRESERVATIONQ3

GLYCEROLQ2

VITRIFICATIONQ2

DESICCATIONQ3

AND ACER MONSPESSULANUMQ3

Abstract

Montpellier Maple (Acer monspessulanum) is one of the broad leaf species grows in semi-arid and cold climate of I.R. Iran. Preservation of this wild woody species has a great importance in natural resource point of view. CRYOPRESERVATION technique is used to preserve seed and vegetative organs under -196oC conditions. In the mentioned temperature, all of the metabolic and physiological activities of the seed or organ almost is stopped. As the result, seed and organ can survive for a long period of time. In order to preserve A. monspessulanum seed under -196oC condition, three pre-CRYOPRESERVATION treatments, PVS2 solution, DESICCATION and 30% GLYCEROL were applied. The treated seeds were transferred into Liquid Nitrogen (LN) and stored for 7 and 30 days respectively. After finishing storage period, the seeds were removed from the LN, imposed to heat shock and transferred between wet-papers in germination boxes. The cryogenic seeds together with control seeds were incubated at +4oC for 105 days to germinate. Mean germination percentage of the cryopreserved seeds was 19.18% and, there were no significant differences between the control and cryopreserved seeds. No adverse effects or abnormalities were observed on seedlings developed from cryopreserved seeds. The results showed that, the long-term preservation of the species, seed in -196oC using suitable pre-CRYOPRESERVATION treatment is possible.

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References

INVESTIGATION OF SUITABLE SEED GERMINATION ENHANCEMENT AND BREAKING SEED DORMANCY TREATMENT OF MONTPELLIER MAPLE (ACER MONOSPESSULANUM L.)

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APA: Copy

HATAMI, FIROUZEH, JEBELI, MARYAM, NADERI SHAHAB, M.A., TABARI, MASOUD,. (2010). CRYOPRESERVATION OF ACER MONSPESSULANUM SEEDS. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, 18(1 (35)), 12-23. SID. https://sid.ir/paper/104650/en

Vancouver: Copy

HATAMI FIROUZEH, JEBELI MARYAM, NADERI SHAHAB M.A., TABARI MASOUD. CRYOPRESERVATION OF ACER MONSPESSULANUM SEEDS. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH[Internet]. 2010;18(1 (35)):12-23. Available from: https://sid.ir/paper/104650/en

IEEE: Copy

FIROUZEH HATAMI, MARYAM JEBELI, M.A. NADERI SHAHAB, MASOUD TABARI, an, “CRYOPRESERVATION OF ACER MONSPESSULANUM SEEDS,” IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, vol. 18, no. 1 (35), pp. 12–23, 2010, [Online]. Available: https://sid.ir/paper/104650/en

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