IDENTIFICATION OF ENTEROPATHOGENIC E. COLI (EPEC) PATHOTYPE USING PCR TECHNIQUE IN UNDER 5- YEARS OLD CHILDREN IN TEHRAN AND EVALUATION OF THEIR ANTIBIOTIC RESISTANCE PATTERNS

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SOLTAN DALLAL MOHAMMAD MEHDI; SHIRAZI MOHAMMAD HASSAN; AKBARI ABOLFAZL; SHARIFI YAZDI MOHAMMAD KAZEM

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Journal: Razi Journal of Medical Sciences Year:2015 | Volume:22 | Issue:137 Page(s): 54-62

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Title

IDENTIFICATION OF ENTEROPATHOGENIC E. COLI (EPEC) PATHOTYPE USING PCR TECHNIQUE IN UNDER 5- YEARS OLD CHILDREN IN TEHRAN AND EVALUATION OF THEIR ANTIBIOTIC RESISTANCE PATTERNS

Author(s)

SOLTAN DALLAL MOHAMMAD MEHDI | SHIRAZI MOHAMMAD HASSAN | AKBARI ABOLFAZL | SHARIFI YAZDI MOHAMMAD KAZEM | Issue Writer Certificate

Keywords

MULTI-ANTIBIOTIC RESISTANCEQ2

ENTEROPATHOGENIC ESCHERICHIA COLI (EPEC)Q2

CHILDHOOD DIARRHEAQ3

Abstract

Background: Enteropathogenic E. coli (EPEC) pathotypes are belonging to enterobacteriaceae family that is known as agent of gastroenteritis and diarrhea in under 5 year old children. This research is aimed to identify of EPEC pathotypes in diarrheal fewer than 5 years old children and study the patterns of antibiotic resistance of these strains in Tehran.Methods: 300 samples were collected from children with diarrhea visited in children Hospital in Tehran. Bacterial isolates confirmed as E. coli species on the basis of standard bacterial and biochemical tests. All isolates were subjected to polymerase chain reaction (PCR) for detection of eaeA (intimin) gene in terms of EPEC pathotypes. Then, antibiogram test for EPEC pathotypes was performed using 16 different antibiotic disces by disc diffusion agar (Kirby-Bauer) method.Results: Among all specimens, 36 (12%) isolates bearing eaeA gene were confirmed as EPEC using PCR. 22 specimens (61.1%) of identified pathotypes were isolated from under 1 year old children. All pathotypes were sensitive to imipenem and meropenem. Antibiotic resistance toward amoxicillin and tetracycline 23 (63.9%) was higher than others. Also 24 isolates (66.7%) showed multi-resistant pattern toward several routine antibiotics.Conclusion: Since EPEC pathotypes are diagnosed as agent of diarrhea in children in our country, identification of these pathotypes showed be made through novel techniques.

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APA: Copy

SOLTAN DALLAL, MOHAMMAD MEHDI, SHIRAZI, MOHAMMAD HASSAN, AKBARI, ABOLFAZL, & SHARIFI YAZDI, MOHAMMAD KAZEM. (2015). IDENTIFICATION OF ENTEROPATHOGENIC E. COLI (EPEC) PATHOTYPE USING PCR TECHNIQUE IN UNDER 5- YEARS OLD CHILDREN IN TEHRAN AND EVALUATION OF THEIR ANTIBIOTIC RESISTANCE PATTERNS. RAZI JOURNAL OF MEDICAL SCIENCES (JOURNAL OF IRAN UNIVERSITY OF MEDICAL SCIENCES), 22(137), 54-62. SID. https://sid.ir/paper/10612/en

Vancouver: Copy

SOLTAN DALLAL MOHAMMAD MEHDI, SHIRAZI MOHAMMAD HASSAN, AKBARI ABOLFAZL, SHARIFI YAZDI MOHAMMAD KAZEM. IDENTIFICATION OF ENTEROPATHOGENIC E. COLI (EPEC) PATHOTYPE USING PCR TECHNIQUE IN UNDER 5- YEARS OLD CHILDREN IN TEHRAN AND EVALUATION OF THEIR ANTIBIOTIC RESISTANCE PATTERNS. RAZI JOURNAL OF MEDICAL SCIENCES (JOURNAL OF IRAN UNIVERSITY OF MEDICAL SCIENCES)[Internet]. 2015;22(137):54-62. Available from: https://sid.ir/paper/10612/en

IEEE: Copy

MOHAMMAD MEHDI SOLTAN DALLAL, MOHAMMAD HASSAN SHIRAZI, ABOLFAZL AKBARI, and MOHAMMAD KAZEM SHARIFI YAZDI, “IDENTIFICATION OF ENTEROPATHOGENIC E. COLI (EPEC) PATHOTYPE USING PCR TECHNIQUE IN UNDER 5- YEARS OLD CHILDREN IN TEHRAN AND EVALUATION OF THEIR ANTIBIOTIC RESISTANCE PATTERNS,” RAZI JOURNAL OF MEDICAL SCIENCES (JOURNAL OF IRAN UNIVERSITY OF MEDICAL SCIENCES), vol. 22, no. 137, pp. 54–62, 2015, [Online]. Available: https://sid.ir/paper/10612/en

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