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Information Journal Paper

Title

Spleen extracellular matrix provides a supportive microenvironment for β,-cell function

Pages

  1159-1165

Abstract

 Objective(s): Type 1 diabetes mellitus is a common autoimmune and multifactorial disorder. Researchers have been interested in making a favorable islet-like tissue model for the treatment of diabetes. The main objective of this study was to determine the effects of the Spleen Extracellular matrix (S-ECM) on the function of the MIN6 cell line (a β,-cell model). Materials and Methods: In this experimental research, Wistar rat Spleens were decellularized by sodium dodecyl sulfate (SDS) and Triton X-100. S-ECM was characterized by histological assessments, scanning electron microscopy, determination of residua DNA, and examination of the mechanical tensile property. Then, MIN6 cells were seeded on S-ECM scaffold. Glucose-stimulated Insulin secretion and mRNA expression of Insulin-related genes were examined to confirm the function of the cells. Results: The main components of S-ECM such as collagen and glycosaminoglycan remained after decellularization. Furthermore, very low residual DNA and appropriate mechanical behavior of S-ECM provided an ideal extracellular microenvironment for the MIN6 cells. GSIS results showed that the seeded cells in S-ECM secreted more Insulin than the traditional two-dimensional (2D) culture. The expression of specific Insulin-related genes such as PDX-1, Insulin, Maf-A, and Glut-2 in the recellularized scaffold was more significant than in the 2D traditional cultured cells. Also, MTT assay results showed that S-ECM were no cytotoxic effects on the MIN6 cells. Conclusion: These results collectively have evidenced that S-ECM is a suitable scaffold for stabilizing artificial pancreatic islands.

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    APA: Copy

    Heidari Moghadam, Abbas, & Asadi Fard, Yousef. (2022). Spleen extracellular matrix provides a supportive microenvironment for β,-cell function. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 25(9), 1159-1165. SID. https://sid.ir/paper/1085969/en

    Vancouver: Copy

    Heidari Moghadam Abbas, Asadi Fard Yousef. Spleen extracellular matrix provides a supportive microenvironment for β,-cell function. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2022;25(9):1159-1165. Available from: https://sid.ir/paper/1085969/en

    IEEE: Copy

    Abbas Heidari Moghadam, and Yousef Asadi Fard, “Spleen extracellular matrix provides a supportive microenvironment for β,-cell function,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 25, no. 9, pp. 1159–1165, 2022, [Online]. Available: https://sid.ir/paper/1085969/en

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