MICROPROPAGATION OF EUCALYPTUS MACULATA FROM MATURE STOCK BY TISSUE CULTURE

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GHAMARI ZARE A.; SEDAGHATI M.; EMAM M.; ASSAREH M.H.; KIAROSTAMI KH.

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Journal Paper

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Journal: Iranian Journal of Forest and Poplar Research Year:2014 | Volume:21 | Issue:3 (53) Page(s): 581-593

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Title

MICROPROPAGATION OF EUCALYPTUS MACULATA FROM MATURE STOCK BY TISSUE CULTURE

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Keywords

EUCALYPTUSQ1

ASEXUAL PROPAGATIONQ2

SHOOTINGQ2

ROOTINGQ2

TISSUE CULTUREQ2

MEDIA CULTUREQ2

Abstract

Due to EUCALYPTUS potential as a fast growing species, its utilization for afforestation and industrial and medical purposes is very important. E. maculata Hook. is an important species among the other eucalypts in respect to some chemical components production and medical utilization. Sexual and ASEXUAL PROPAGATION of this species is difficult, for this reason the trial was conducted to investigate its propagation by TISSUE CULTURE, using micro mature stocks.The produced explants from the mature stocks, were placed on MS medium (Murashige and Skooge) treated by 1/2 nitrate and various growth regulators such as Kin (Kinetin), BAP (Benzylamino purine), IBA (Indole-3-acetic acid), 2ip (2-Isopentyladenine), TDZ (Tidiazorun) and NAA (Naphtalen Acetic Acid) at different levels of concentration. After two months, growth indexes, including shoot number, shoot height, root number, root length, bud number and greenness rate were measured and recorded. Results showed that the best SHOOTING treatment was IBA (0.01 mgl-1), BAP (0.3 mgl-1) and 2ip (0.5 mgl-1), in which average shoot number, shoot height and greenness rate were 4.63, 2.73 cm and 3.75, respectively, whereas the best ROOTING treatment was NAA (0.5 mgl-1) plus IBA (0.5 mgl-1), in which root length was 0.2 cm. Finally, the micropropagated plantlets were transferred to greenhouse to pass their adaptation process.

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APA: Copy

GHAMARI ZARE, A., SEDAGHATI, M., EMAM, M., ASSAREH, M.H., & KIAROSTAMI, KH.. (2014). MICROPROPAGATION OF EUCALYPTUS MACULATA FROM MATURE STOCK BY TISSUE CULTURE. IRANIAN JOURNAL OF FOREST AND POPLAR RESEARCH, 21(3 (53)), 581-593. SID. https://sid.ir/paper/111199/en

Vancouver: Copy

GHAMARI ZARE A., SEDAGHATI M., EMAM M., ASSAREH M.H., KIAROSTAMI KH.. MICROPROPAGATION OF EUCALYPTUS MACULATA FROM MATURE STOCK BY TISSUE CULTURE. IRANIAN JOURNAL OF FOREST AND POPLAR RESEARCH[Internet]. 2014;21(3 (53)):581-593. Available from: https://sid.ir/paper/111199/en

IEEE: Copy

A. GHAMARI ZARE, M. SEDAGHATI, M. EMAM, M.H. ASSAREH, and KH. KIAROSTAMI, “MICROPROPAGATION OF EUCALYPTUS MACULATA FROM MATURE STOCK BY TISSUE CULTURE,” IRANIAN JOURNAL OF FOREST AND POPLAR RESEARCH, vol. 21, no. 3 (53), pp. 581–593, 2014, [Online]. Available: https://sid.ir/paper/111199/en

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