INVESTIGATION ON POSSIBILITY OF CRYOPRESERVATION OF EUCALYPTUS MICROTHECA SEEDS

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HATAMI F.; NADERI SHAHAB M.A.; JEBELLI M.; GHAMARI ZARE A.; TABARI M.; ASSAREH M.H.

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Journal: Iranian Journal of Forest and Poplar Research Year:2010 | Volume:17 | Issue:4 (38) Page(s): 627-636

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Information Journal Paper

Title

INVESTIGATION ON POSSIBILITY OF CRYOPRESERVATION OF EUCALYPTUS MICROTHECA SEEDS

Author(s)

Keywords

EUCALYPTUS MICROTHECAQ3

CRYOPRESERVATIONQ3

GLYCEROL 30%Q3

PLANT VITRIFICATION SOLUTION 2 (PVS2)Q3

DESICCATIONQ3

LIQUID NITROGENQ3

Abstract

The aim of this study was to investigate the possibility of CRYOPRESERVATION of EUCALYPTUS MICROTHECA seeds at -196ºC, using LIQUID NITROGEN (LN). Before transferring the seeds into LN, three pre-treatments including Plant Vitrification Solution 2 (PVS2), DESICCATION and GLYCEROL 30% was applied. The treated seeds transferred into LN for period of one week. The seeds removed from the LN, subjected to heat shock (+42ºC) for 2 minutes, blotted and transferred both onto moist paper in petri dishes and pots, filled with soil and pit moss. The petri dishes and pots, transferred into germinator (+24ºC) or greenhouse (+20ºC), respectively. Subsequent of CRYOPRESERVATION period, seed germination and recovery was high, there were no significant differences between cryopreserved and control seeds in germinator and greenhouse conditions. Furthermore, there were no adverse effect or abnormality observed in seedlings developed from cryopreserved seeds. Results showed that, long-term preservation of E. microtheca seeds under LN (cryopreservation) is promising.

Cites

SEED GERMINATION OF LILIUM LEDEBOURII (BAKER) BOISS. AFTER CRYOPRESERVATION

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APA: Copy

HATAMI, F., NADERI SHAHAB, M.A., JEBELLI, M., GHAMARI ZARE, A., TABARI, M., & ASSAREH, M.H.. (2010). INVESTIGATION ON POSSIBILITY OF CRYOPRESERVATION OF EUCALYPTUS MICROTHECA SEEDS. IRANIAN JOURNAL OF FOREST AND POPLAR RESEARCH, 17(4 (38)), 627-636. SID. https://sid.ir/paper/111431/en

Vancouver: Copy

HATAMI F., NADERI SHAHAB M.A., JEBELLI M., GHAMARI ZARE A., TABARI M., ASSAREH M.H.. INVESTIGATION ON POSSIBILITY OF CRYOPRESERVATION OF EUCALYPTUS MICROTHECA SEEDS. IRANIAN JOURNAL OF FOREST AND POPLAR RESEARCH[Internet]. 2010;17(4 (38)):627-636. Available from: https://sid.ir/paper/111431/en

IEEE: Copy

F. HATAMI, M.A. NADERI SHAHAB, M. JEBELLI, A. GHAMARI ZARE, M. TABARI, and M.H. ASSAREH, “INVESTIGATION ON POSSIBILITY OF CRYOPRESERVATION OF EUCALYPTUS MICROTHECA SEEDS,” IRANIAN JOURNAL OF FOREST AND POPLAR RESEARCH, vol. 17, no. 4 (38), pp. 627–636, 2010, [Online]. Available: https://sid.ir/paper/111431/en

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