Design and fabrication of a microfluidic chip for oocyte denudation.

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Safaefar F.; Karamdel J.; Veladi H.; Fallah Tafti M.; Maleki M.

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Journal Paper

Paper Information

Journal: TABRIZ JOURNAL OF ELECTRICAL ENGINEERING Year:2022 | Volume:52 | Issue:3 Page(s): 189-194

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Information Journal Paper

Title

Design and fabrication of a microfluidic chip for oocyte denudation.

Author(s)

Keywords

Assisted Reproductive Technology (ART)Q4

DenudationQ4

in vitro maturation (IVM)Q4

MicrofluidicsQ4

lab-on-chipQ4

Abstract

Intracytoplasmic sperm injection (ICSI) is one of the most successful techniques of Assisted Reproductive Technology (ART) and is mostly in use for the treatment of infertility with male factors. In this method, before injecting sperm into the intracytoplasmic of the oocyte, cumulus cells around the oocyte must be stripped to facilitate the injection process. To achieve this, both enzymatic and mechanical methods are used in embryological laboratories for Denudation, which has major deficiencies, including the possibility of damaging the oocyte prior to the injection process. In this research, a microfluidic-based device is introduced for the separation of cumulus cells around the oocyte with minimum manual operations. The results prove high efficiency, and non-destructive Denudation of the oocyte with the reduced amount of culture medium leads to the low-cost preparation process of oocytes. The process can also be integrated with ICSI chips under development and will be reported shortly.

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