AGROBACTERIUM-MEDIATED TRANSFORMATION OF WDREB2 TRANSCRIPTION FACTOR TO WHEAT FOR IMPROVEMENT OF TOLERANCE TO ABIOTIC STRESSES

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

YARIZADE A.; NIAZI A.; SAZEGARI S.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: MODERN GENETICS JOURNAL (MGJ) Year:2014 | Volume:8 | Issue:4 (35) Page(s): 357-364

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

633

Download:

Cites:

Information Journal Paper

Title

AGROBACTERIUM-MEDIATED TRANSFORMATION OF WDREB2 TRANSCRIPTION FACTOR TO WHEAT FOR IMPROVEMENT OF TOLERANCE TO ABIOTIC STRESSES

Author(s)

YARIZADE A. | NIAZI A. | SAZEGARI S. | Issue Writer Certificate

Keywords

ENVIRONMENTAL STRESSESQ2

GENE TRANSFORMATIONQ2

REAL TIME PCRQ2

WDREB2 TRANSCRIPTION FACTORQ2

WHEATQ2

Abstract

Abiotic stresses such as drought, cold and salinity have negative effects on yield and growth plant, so tolerance to them has been main target of plant breeders. DREB transcription factors are member of AP2/ERF family. Analysis of promoter region of genes which responsive to drought and low temperature stresses and ABA-independent, led to the discovery of 9pb region with TACCGACAT sequence that named DER. Proteins which are encoded by DREB genes have attached to this sequence and regulate gene expression. WDREB2 or TaDREB gene expression is induced by drought, cold, abscisic acid and salinity stresses. This gene was transferred to WHEAT using Agrobacterium-mediated GENE TRANSFORMATION method. Molecular analysis with PCR technique confirms that 20 plants out of 40 plants are transgenic. Also gene expression analyses with Real-Time PCR Technique have showed that the most part of transgenic plants have increased expression between 2 to 19 times, more than non-transgenic plants. This increasing of expression which demonstrate the efficiency of 35S promoter in monocot plants, can lead to the raising of expression in downstream genes and enhancement of tolerance to environmental abiotic stresses.

Cites

No record.

References

No record.

Cite

APA: Copy

YARIZADE, A., NIAZI, A., & SAZEGARI, S.. (2014). AGROBACTERIUM-MEDIATED TRANSFORMATION OF WDREB2 TRANSCRIPTION FACTOR TO WHEAT FOR IMPROVEMENT OF TOLERANCE TO ABIOTIC STRESSES. MODERN GENETICS JOURNAL (MGJ), 8(4 (35)), 357-364. SID. https://sid.ir/paper/116447/en

Vancouver: Copy

YARIZADE A., NIAZI A., SAZEGARI S.. AGROBACTERIUM-MEDIATED TRANSFORMATION OF WDREB2 TRANSCRIPTION FACTOR TO WHEAT FOR IMPROVEMENT OF TOLERANCE TO ABIOTIC STRESSES. MODERN GENETICS JOURNAL (MGJ)[Internet]. 2014;8(4 (35)):357-364. Available from: https://sid.ir/paper/116447/en

IEEE: Copy

A. YARIZADE, A. NIAZI, and S. SAZEGARI, “AGROBACTERIUM-MEDIATED TRANSFORMATION OF WDREB2 TRANSCRIPTION FACTOR TO WHEAT FOR IMPROVEMENT OF TOLERANCE TO ABIOTIC STRESSES,” MODERN GENETICS JOURNAL (MGJ), vol. 8, no. 4 (35), pp. 357–364, 2014, [Online]. Available: https://sid.ir/paper/116447/en

Related Journal Papers