SELECTION OF SUITABLE REFERENCE GENES FOR REAL-TIME PCR IN WHEAT FLAG LEAVES UNDER DROUGHT STRESS

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MALEKI F.; ZAREI KH.; FOTOVAT R.; SHOBBAR Z.S.

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Paper Information

Journal: MODERN GENETICS JOURNAL (MGJ) Year:2016 | Volume:11 | Issue:3 Page(s): 469-473

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Information Journal Paper

Title

SELECTION OF SUITABLE REFERENCE GENES FOR REAL-TIME PCR IN WHEAT FLAG LEAVES UNDER DROUGHT STRESS

Author(s)

MALEKI F. | ZAREI KH. | FOTOVAT R. | SHOBBAR Z.S. | Issue Writer Certificate

Keywords

BESTKEEPERQ2

DROUGHTQ1

REFERENCE GENESQ1

NORMFINDERQ1

REAL-TIME PCRQ1

Abstract

many studies on the defence and stress mechanisms in plants are based on gene expression. The stability of the REFERENCE GENES expression used in REAL-TIME PCR is a further major step in data normalization. For validation of REFERENCE GENES as internal control in wheat flag leaves under DROUGHT stress, the expression stability of eight candidate genes, including ribosomal RNA subunit (18S rRNA), ubiquitin (UBQ and WUB), Actin (ACT), glyseraldehyd-3-phosphate dehydrogenase (GAPDH), RNase L inhibitor-like protein (RLI), beta-tubulin 4 (bTUB) and ATP-dependent 26s proteasome regulatory subunit (Ta.22845), was evaluated using NORMFINDER and BESTKEEPER softwaresResults indicated that RLI and UBQ were the most stable genes, whereas 18S rRNA was least stable gene among the eight tested genes for the normalization of gene expression in wheat flag leaf under DROUGHT and normal conditions.

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APA: Copy

MALEKI, F., ZAREI, KH., FOTOVAT, R., & SHOBBAR, Z.S.. (2016). SELECTION OF SUITABLE REFERENCE GENES FOR REAL-TIME PCR IN WHEAT FLAG LEAVES UNDER DROUGHT STRESS. MODERN GENETICS JOURNAL (MGJ), 11(3), 469-473. SID. https://sid.ir/paper/116502/en

Vancouver: Copy

MALEKI F., ZAREI KH., FOTOVAT R., SHOBBAR Z.S.. SELECTION OF SUITABLE REFERENCE GENES FOR REAL-TIME PCR IN WHEAT FLAG LEAVES UNDER DROUGHT STRESS. MODERN GENETICS JOURNAL (MGJ)[Internet]. 2016;11(3):469-473. Available from: https://sid.ir/paper/116502/en

IEEE: Copy

F. MALEKI, KH. ZAREI, R. FOTOVAT, and Z.S. SHOBBAR, “SELECTION OF SUITABLE REFERENCE GENES FOR REAL-TIME PCR IN WHEAT FLAG LEAVES UNDER DROUGHT STRESS,” MODERN GENETICS JOURNAL (MGJ), vol. 11, no. 3, pp. 469–473, 2016, [Online]. Available: https://sid.ir/paper/116502/en

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