PHYSIOLOGICAL EVALUATION, ISOLATION AND GENE EXPRESSION INVESTIGATION OF NADP- MALIC ENZYME UNDERSALINITY STRESS IN AELUROPUS LITTORALIS

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YOUNESI MELERDI E.; NEMATZADEH GH.; SHOKRI E.

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Journal: MODERN GENETICS JOURNAL (MGJ) Year:2017 | Volume:12 | Issue:3 Page(s): 463-476

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Title

PHYSIOLOGICAL EVALUATION, ISOLATION AND GENE EXPRESSION INVESTIGATION OF NADP- MALIC ENZYME UNDERSALINITY STRESS IN AELUROPUS LITTORALIS

Author(s)

YOUNESI MELERDI E. | NEMATZADEH GH. | SHOKRI E. | Issue Writer Certificate

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Abstract

In the present study, to investigate the photosynthetic mechanism of this plant under salinity stree, to levels of 0 (control) and 600 mM NaCl were applied for 10 days then plant responses in physiological and molecular levels were assessed. For this end, the coding sequence of this gene was isolated from Aeluropus littoralis for first time and the bioinformatic investigation were done. Results showed that the chlorophyll amount, the starch content and Sodium excretion of leaves in 600 600 mM NaCl were significantly (P<0.05) different from the control. After isolation, the coding sequence of nadp - me gene was recorded to gene bank under accession number of KP122942.1. The nucleotide and protein blasts showed maximum indentity between isolated sequence and sequence of NADP-ME of halophyte Megathyrsus maximus. Also, the protein sequence analysis confirmed that isolated sequence with length of 74 amino acids is a portion from NAD (P)-binding domain. The results of relative expression analysis showed that NADP-ME gene expression in 600 mM NaCl was 2.19 times more than control. According to obtained results in present study, it can be stated that increased expression of nadp-me gene could have effective role in resistance to salinity stress in Aeluropus littoralis.

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APA: Copy

YOUNESI MELERDI, E., NEMATZADEH, GH., & SHOKRI, E.. (2017). PHYSIOLOGICAL EVALUATION, ISOLATION AND GENE EXPRESSION INVESTIGATION OF NADP- MALIC ENZYME UNDERSALINITY STRESS IN AELUROPUS LITTORALIS. MODERN GENETICS JOURNAL (MGJ), 12(3 ), 463-476. SID. https://sid.ir/paper/116527/en

Vancouver: Copy

YOUNESI MELERDI E., NEMATZADEH GH., SHOKRI E.. PHYSIOLOGICAL EVALUATION, ISOLATION AND GENE EXPRESSION INVESTIGATION OF NADP- MALIC ENZYME UNDERSALINITY STRESS IN AELUROPUS LITTORALIS. MODERN GENETICS JOURNAL (MGJ)[Internet]. 2017;12(3 ):463-476. Available from: https://sid.ir/paper/116527/en

IEEE: Copy

E. YOUNESI MELERDI, GH. NEMATZADEH, and E. SHOKRI, “PHYSIOLOGICAL EVALUATION, ISOLATION AND GENE EXPRESSION INVESTIGATION OF NADP- MALIC ENZYME UNDERSALINITY STRESS IN AELUROPUS LITTORALIS,” MODERN GENETICS JOURNAL (MGJ), vol. 12, no. 3 , pp. 463–476, 2017, [Online]. Available: https://sid.ir/paper/116527/en

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